Anti-DRP1 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O00429

Core Product Specifications and Parameters

Biological Background: DNM1L Function and Localization

• Mitochondrial and peroxisomal division: Functions in mitochondrial and peroxisomal division, mediating membrane fission via GTP hydrolysis-dependent constriction.
  Related references:
  PMID:11514614, PMID:12499366, PMID:17301055, PMID:23530241, PMID:23584531, PMID:33850055

• Recruitment to scission sites: Specifically recruited to mitochondrial membranes by receptors MFF, MIEF1, and MIEF2 in a GTP-dependent manner; GTP hydrolysis triggers dissociation and ring formation.
  Related references:
  PMID:23283981, PMID:23921378, PMID:29899447

• Mitochondrial fission during mitosis and neuroprotection: Plays a critical role in mitochondrial fission during mitosis and is essential for survival of postmitotic neurons, such as Purkinje cells, by suppressing oxidative damage.
  Related references:
  PMID:19411255, PMID:26992161, PMID:27301544

• Brain development: Required for normal brain development, including cerebellum formation.
  Related references:
  PMID:17460227, PMID:26992161, PMID:27145208

• Programmed necrosis: Required for programmed necrosis execution.
  Related references:
  PMID:22265414

• Apoptosis: Facilitates cytochrome c release and caspase activation during apoptosis; requirement is cell type- and physiological cue-dependent.

• Circadian regulation: Rhythmic control of its activity via phosphorylation at Ser-637 is essential for circadian mitochondrial ATP production.
  Related references:
  PMID:29478834

• Subcellular localization and isoform expression: Localizes to the cytoplasm, mitochondrial outer membrane, peroxisome, Golgi, clathrin-coated pits, and synaptic vesicles. Ubiquitously expressed, with highest levels in skeletal muscle, heart, kidney, and brain; specific isoforms are enriched in brain (isoform 1), testis (isoform 2), skeletal muscle (isoform 3), and neurons (isoform 6).

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~82 kDa; validate band specificity using appropriate positive and negative controls or siRNA knockdown.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), co-stain with organelle markers (e.g., Tom20 for mitochondria) to confirm expected localization patterns.
• Since the immunogen spans the C-terminal region (amino acids 437–736), the antibody may recognize multiple DRP1 isoforms; verify isoform detection in the relevant biological system.
• Cross-reactivity with mouse and rat is reported; confirm reactivity using species‑matched control tissues or lysates.
• For ELISA applications, the antibody can be used for titer determination or as a capture/detection reagent after validation with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, technical services, and consulting from concept to clinic. Our DRP1 antibody supports key research areas in mitochondrial dynamics, neurodegeneration, and apoptosis. We offer validated antibody pairs, bulk ancillary reagents, and tailored sourcing solutions for rare or specialized targets to accelerate your diagnostic and research workflows.