Anti-DRP1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IF-P, ELISA - O00429

Core Product Specifications and Parameters

Biological Background: DRP1 (DNM1L) Function and Localization

• Dynamin-1-like protein (DNM1L), also known as DRP1, DVLP, or Dymple, is a member of the dynamin superfamily of large GTPases that mediate membrane remodeling events.
• Essential for mitochondrial and peroxisomal division by oligomerizing into helical structures that constrict and sever membranes through GTP hydrolysis.
  Related references: PMID:11514614 PMID:12499366 PMID:17301055
• Recruitment to mitochondrial scission sites is directed by adaptor proteins MFF, MIEF1, and MIEF2; GTP binding triggers recruitment, while hydrolysis promotes disassembly and ring closure for fission.
  Related references: PMID:23283981 PMID:23921378 PMID:29899447
• Functions downstream of PINK1 and PRKN in mitophagy, and supports mitochondrial division during mitosis and neuronal survival, with roles in apoptosis and necrotic cell death.
  Related references: PMID:32484300 PMID:19411255 PMID:26992161 PMID:27301544 PMID:22265414
• Participates in endocytosis and synaptic vesicle dynamics, where BCL2L1 stimulates its GTPase activity, linking mitochondrial dynamics to neurotransmitter release.
  Related references: PMID:9570752 PMID:23792689 PMID:17015472
• Circadian phosphorylation at Ser‑637 controls rhythmic mitochondrial ATP production, underscoring a role in daily energy metabolism.
  Related references: PMID:29478834
• Localizes to cytosol, mitochondrial outer membrane, peroxisomes, Golgi apparatus, clathrin‑coated pits, and synaptic vesicles, reflecting its multifaceted cellular functions.
• Ubiquitously expressed with highest levels in skeletal muscle, heart, kidney, and brain; multiple isoforms display tissue‑specific patterns, including a brain‑specific isoform 1 and neuronal isoform 6.
• Post‑translational modifications include phosphorylation, acetylation, S‑nitrosylation, and ubiquitination, while disease variants (e.g., OPA5) connect DNM1L mutations to human pathologies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal peptide (aa 561‑670) of human DRP1, which is conserved across human, mouse, rat, and monkey; pre‑adsorbed or knockout validation can confirm specificity in these species.
• For Western blotting, expect a band near 82 kDa; be aware that post‑translational modifications and alternative splicing may produce additional bands.
• In immunoprecipitation (IP) and immunofluorescence (IF/ICC, IF‑P), consider that DRP1 dynamically shuttles between cytosolic and membrane‑associated pools—use appropriate fixation and permeabilization conditions.
• The monoclonal nature of this rabbit antibody helps ensure consistent lot‑to‑lot performance; however, we recommend pilot testing with relevant positive and negative controls in your specific tissue or cell line.
• Because DRP1 interacts with multiple receptors and adaptors, co‑IP experiments may require careful optimization of lysis and washing conditions to preserve complexes.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing—all designed to support diagnostic development from concept to clinic. This anti‑DRP1 rabbit monoclonal antibody provides a robust tool for investigating mitochondrial dynamics, neurodegenerative diseases, and metabolic disorders, complementing our comprehensive portfolio of cell biology and disease‑relevant reagents. Let CamelBio streamline your procurement with reliable, high‑quality raw materials and expert technical guidance.