Anti-DROSHA Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9NRR4

Core Product Specifications and Parameters

Biological Background: DROSHA Function and Localization

• DROSHA (Ribonuclease 3) is a nuclear protein also known as RNASEN, RN3, or RNase III, encoded by the DROSHA gene. It is a member of the ribonuclease III family of double-stranded RNA-specific endoribonucleases.
• As the catalytic subunit of the microprocessor complex, DROSHA processes primary miRNA transcripts (pri-miRNAs) into ~70-nucleotide precursor miRNAs (pre-miRNAs) in the nucleus, initiating the canonical miRNA biogenesis pathway.
• It cleaves both the 3' and 5' strands of the stem-loop structure in pri-miRNAs at a defined distance from the junction, releasing hairpin pre-miRNAs for further processing by DICER in the cytoplasm.
• Beyond miRNA maturation, DROSHA participates in pre-ribosomal RNA processing and plays a role in the formation of GW/P-bodies, linking it to RNA metabolism and gene silencing.
• Subcellularly, DROSHA predominantly localizes to the nucleus, with enrichment in the nucleolus, but can also be detected in the cytoplasm under certain conditions.
• The protein is ubiquitously expressed and subject to alternative splicing and post-translational modifications including phosphorylation, which may regulate its activity.
• Keywords associated with DROSHA include: Endonuclease, Hydrolase, RNA-binding, Magnesium/Manganese metal-binding, and RNA-mediated gene silencing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100). For applications like Western blot, the full-length protein migrates at ~159 kDa; ensure adequate transfer and use appropriate molecular weight markers.
• For immunofluorescence/ICC, fixation and permeabilization conditions should be optimized as the target is predominantly nuclear; consider using a nuclear counterstain to confirm localization.
• ELISA performance can be verified using recombinant DROSHA protein or synthetic peptide; titration of the antibody is recommended.
• Verify reactivity in your specific human-derived sample system, as expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research institutes, providing a comprehensive range of IVD raw materials and technical support from concept to commercialization. Our catalog includes validated antibody pairs, optimized monoclonal antibodies like this DROSHA clone, and bulk ancillary reagents for nucleic acid and protein detection. Whether you need a rare target such as DROSHA for miRNA research or established biomarkers, our sourcing expertise streamlines your assay development and manufacturing.