Anti-DRG1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y295

Core Product Specifications and Parameters

Biological Background: DRG1 Function and Localization

• DRG1 (Developmentally-regulated GTP-binding protein 1), also known as NEDD3, catalyzes the hydrolysis of GTP to GDP, functioning as a translation factor GTPase. Related references: PMID:23711155, PMID:29915238, PMID:37179472
• Its intrinsic GTPase activity is stimulated by the binding partner ZC3H15/DFRP1, which enhances potassium ion affinity. Related references: PMID:23711155
• Hydroxylation at Lys-22 by JMJD7 enables RNA binding and suggests a role in ribosomal translation. Related references: PMID:19819225, PMID:29915238
• DRG1 binds to microtubules and promotes their polymerization and stability, crucial for mitotic spindle assembly during prophase to anaphase; this function is independent of GTPase activity. Related references: PMID:28855639
• DRG1 localizes to both the nucleus and cytoplasm, reflecting its diverse roles in cell division and translation.
• Tissue expression is highest in skeletal muscle, heart, and kidney; moderate in liver, placenta, and brain; and low in colon, thymus, spleen, small intestine, lung, and leukocytes.
• Post-translational modifications include acetylation, hydroxylation, phosphorylation, and ubiquitination; mutations in DRG1 are associated with dwarfism and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen comprises the central region (aa 158-367) of human DRG1, which may confer reactivity across human, mouse, and rat orthologs.
• For Western blotting, consider an initial dilution range of 1:500–1:2000 and validate with appropriate positive and negative controls.
• For ELISA, pair with a suitable secondary antibody and optimize antigen coating conditions for your specific assay format.
• Since DRG1 is expressed in both nuclear and cytoplasmic fractions, verify localization patterns in your cell or tissue system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focusing on DRG1 and its roles in cell cycle regulation, microtubule dynamics, and translation, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support assay development and clinical translation. Whether your project requires routine immunoassays or customized rare-target raw material sourcing, CamelBio streamlines the procurement process.