Anti-DREB1B Rabbit Polyclonal Antibody for WB and ELISA - P93835

Core Product Specifications and Parameters

Biological Background: DREB1B Function and Localization

• DREB1B (also known as CBF1 or ERF029) is a member of the C-repeat/dehydration-responsive element-binding factor (CBF/DREB) subfamily of AP2/ERF transcription factors.
• It functions as a transcriptional activator that specifically binds to the DNA sequence 5'-[AG]CCGAC-3', known as the C-repeat/dehydration-responsive element (DRE).
• Binding to the C-repeat/DRE element mediates cold-inducible transcription, playing a key role in freezing tolerance and cold acclimation.
• The protein localizes to the nucleus, consistent with its role as a transcription factor.
• Key biological keywords include activator, DNA-binding, nucleus, stress response, transcription, and transcription regulation.
• The canonical protein has a predicted molecular weight of approximately 23.8 kDa (based on UniProt data).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50-150 of DREB1B. This region may exhibit homology with other CBF family members; consider sequence alignment if cross-reactivity is a concern.
• For western blotting (WB), validate the antibody using samples from cold‑stressed Arabidopsis tissues where DREB1B expression is induced.
• In ELISA applications, optimize coating and detection conditions with recombinant DREB1B or cell lysates from appropriate Arabidopsis backgrounds.
• The antibody is polyclonal; for quantitative studies, confirm batch‑to‑batch consistency and consider an appropriate secondary antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For plant stress biology and transcriptional regulation research, our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. If your project requires rare‑target antibodies or scalable raw‑material supply for assay development, CamelBio’s network can help bridge the gap.