Anti-DRD4 Polyclonal Antibody for WB, IHC-P, ELISA - P21917

Core Product Specifications and Parameters

Biological Background: DRD4 Function and Localization

• DRD4, also known as D(2C) dopamine receptor or Dopamine D4 receptor, is a G protein-coupled receptor encoded by the DRD4 gene.
• It mediates dopamine signaling in the mesolimbic system, regulating emotion and complex behavior. Agonist binding by dopamine, epinephrine, norepinephrine, and synthetic drugs triggers G protein-mediated inhibition of adenylyl cyclase. Related references: PMID:16423344 PMID:27659709 PMID:29051383
• DRD4 modulates the circadian rhythm of contrast sensitivity by regulating rhythmic expression of NPAS2 in retinal ganglion cells.
• It localizes predominantly to the cell membrane as a multi-pass transmembrane protein.
• Highly expressed in retina; detected at much lower levels in brain regions including amygdala, thalamus, hypothalamus, cerebellum, and pituitary.
• Post-translational modifications include glycosylation, palmitoylation, disulfide bonds, and ubiquitination; the receptor also contains metal-binding and sodium-binding sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within the N-terminal/transmembrane region (amino acids 100-200) of human DRD4. Confirm specificity using peptide blocking or knockout/knockdown controls.
• For Western blotting, the predicted molecular weight is 44 kDa, but post-translational modifications may cause slight band shifts. Use appropriate loading controls.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary. Validate staining in known positive tissues such as retina or brain sections.
• The antibody cross-reacts with mouse and rat based on sequence homology; verify performance in your target species.
• For ELISA, optimize coating conditions and check for background due to polyclonal nature.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers and research institutes with a complete range of IVD raw materials, technical services, and consulting. For neuroscience studies on the dopaminergic system, we offer validated antibody pairs, optimized polyclonals like this anti-DRD4 antibody, and bulk ancillary reagents. Our global sourcing networks also support custom development and rare-target procurement to streamline your assay development from concept to clinic.