Anti-DRD1 Rabbit Polyclonal Antibody for WB and ELISA - P21728

Core Product Specifications and Parameters

Biological Background: DRD1 Function and Localization

• DRD1 (D(1A) dopamine receptor) is a G protein-coupled receptor that mediates dopamine signaling by activating adenylyl cyclase through G proteins, increasing intracellular cAMP levels.
• The receptor forms heterotetramers with DRD3, which enhances beta-arrestin recruitment and contributes to the regulation of locomotor activity.
• DRD1 is primarily expressed in the central nervous system, with high levels detected in the caudate, nucleus accumbens, and olfactory tubercle.
• Subcellularly, DRD1 localizes to the cell membrane, endoplasmic reticulum membrane, and specific neuronal compartments including the cilium membrane, dendrites, and dendritic spines.
• As a transmembrane protein, it undergoes post-translational modifications such as glycosylation, palmitoylation, and formation of disulfide bonds, which are critical for proper folding and membrane trafficking.
• The receptor belongs to the dopamine receptor family and is a model system for understanding GPCR signaling and synaptic transmission.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 338-446) of human DRD1, suggesting the antibody will recognize epitopes in this domain; consider this for experimental design.
• For Western blot, a band around 49 kDa is expected; use a 10% SDS-PAGE gel and validate in brain tissue lysates from human or rat origin, given the reported cross-reactivity.
• In ELISA applications, pair with a suitable detection antibody and include appropriate controls using recombinant DRD1 protein or peptide standards.
• As a polyclonal antibody, it may show additional bands in WB; optimize blocking and dilution conditions to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with validated reagents. For neuroscience and GPCR signaling research, we offer this anti-DRD1 polyclonal antibody and can supply matched antibody pairs, recombinant proteins, and ancillary reagents to streamline your assay development. Leverage our one-stop platform to source rare target reagents and accelerate your diagnostic projects from concept to clinic.