Anti-DRAP1 Polyclonal Antibody for WB and ELISA - Q14919

Core Product Specifications and Parameters

Biological Background: DRAP1 Function and Localization

• DRAP1 (Dr1-associated protein 1), also known as negative cofactor 2-alpha (NC2-alpha), is a transcriptional corepressor that forms a heterodimer with DR1.
• The DR1/DRAP1 heterodimer binds to TATA-box binding protein (TBP) to repress both activated and basal transcription of class II genes by inhibiting the assembly of transcription-competent complexes (preventing association of TFIIA and/or TFIIB with TBP).
• DRAP1 can also bind DNA independently, suggesting an additional role in gene regulation.
• The protein localizes to the nucleus, consistent with its function in transcriptional regulation.
• DRAP1 undergoes alternative splicing and phosphorylation, as indicated by UniProt keywords.
• It is ubiquitously expressed, with highest levels in adult testis, heart, skeletal muscle, pancreas, and brain, as well as fetal brain, liver, and kidney.
• The predicted molecular weight of DRAP1 is approximately 22 kDa (UniProt: 22350 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 50–150) encompasses the conserved histone fold domain, which may contribute to the observed cross-reactivity with mouse and rat DRAP1.
• For Western blot, expect a band around 22 kDa; nuclear extracts or whole-cell lysates from human, mouse, or rat cells/tissues are suitable starting samples.
• In ELISA, consider using the antibody for antigen capture or detection, and validate with appropriate positive (e.g., recombinant DRAP1) and negative controls.
• As DRAP1 is a nuclear protein, ensure proper sample preparation (e.g., nuclear extraction or lysis buffer optimization) to maximize detection sensitivity.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research institutes, and clinical laboratories a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For transcription regulation research, we streamline sourcing of DRAP1 and related transcriptional corepressor antibodies, supported by technical expertise to accelerate your assay development from concept to clinic.