Anti-DR5/TRAILR2/TNFRSF10B Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O14763

Core Product Specifications and Parameters

Biological Background: DR5/TRAILR2/TNFRSF10B Function and Localization

• DR5 (Death receptor 5), also designated TRAILR2 and CD262, is a member of the tumor necrosis factor receptor superfamily (TNFRSF) encoded by the TNFRSF10B gene. It is a key mediator of extrinsic apoptosis.
• DR5 serves as the receptor for the cytotoxic ligand TNFSF10/TRAIL. Ligand binding recruits FADD and caspase-8 to form the death-inducing signaling complex (DISC), leading to caspase activation and programmed cell death. Related references: PMID:10549288
• Beyond apoptosis, DR5 can activate NF-κB, suggesting additional roles in inflammation and cell survival.
• DR5 is a transmembrane glycoprotein located primarily at the cell surface, featuring a signal peptide, extracellular cysteine-rich repeats, and a cytoplasmic death domain.
• The protein is widely expressed in human tissues, with notable enrichment in tumor cell lines (e.g., HeLaS3, K-562, SW480) and organs such as heart, liver, and pancreas; it is undetectable in the brain.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, likely essential for receptor stability and function.
• Alternative splicing of TNFRSF10B yields multiple transcript variants, potentially producing isoforms with differing regulatory properties.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 341-440 of human DR5. This region lies in the intracellular domain; therefore, the antibody is expected to detect both full-length and any C-terminal-truncated variants in applications requiring intracellular access.
• For Western blot (WB), a band at approximately 48 kDa is anticipated under reducing conditions. Consider using protease inhibitors during lysis to prevent degradation.
• In immunohistochemistry (IHC-P), antigen retrieval using citrate buffer (pH 6) or EDTA (pH 9) may enhance epitope exposure; optimal conditions should be determined experimentally.
• For immunofluorescence staining (IF/ICC), cell permeabilization (e.g., 0.1% Triton X-100) is recommended to allow antibody penetration to the intracellular epitope.
• The reported cross-reactivity with mouse and rat implies sequence homology in the immunogen region; however, signal intensity may vary—validate in relevant model systems.
• As an ELISA-ready reagent, this antibody can be used for capture or detection in sandwich immunoassays—titrate against recombinant DR5 protein for best performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-DR5 antibody exemplifies our commitment to supporting apoptosis and cancer research with well-characterized monoclonal antibodies. Beyond this product, we supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development and commercialization.