Anti-DR4 Rabbit Monoclonal Antibody for FC, ELISA - O00220

Core Product Specifications and Parameters

Biological Background: DR4 Function and Localization

• Receptor identity: TNFRSF10A (Death receptor 4, TRAIL-R1) is a member of the tumor necrosis factor receptor superfamily, characterized by an extracellular domain containing cysteine-rich repeats.
• Apoptosis induction: DR4 serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. Upon ligand binding, the adapter molecule FADD recruits caspase-8 to form the death-inducing signaling complex (DISC), leading to proteolytic activation of caspase-8 and initiation of the apoptotic cascade (PubMed:19090789, PubMed:26457518, PubMed:38532423). Related references: PMID:19090789, PMID:26457518, PMID:38532423
• NF-kappa-B activation: In addition to apoptosis, DR4 promotes the activation of the NF-kappa-B signaling pathway, contributing to inflammatory and survival responses (PubMed:9430227). Related references: PMID:9430227
• Subcellular localization: The receptor is primarily localized to the cell membrane and membrane rafts, but it can also be detected in the cytoplasm/cytosol.
• Tissue expression: DR4 is widely expressed, with notably high levels in the spleen, peripheral blood leukocytes, small intestine, thymus, and certain cancer cell lines (K-562, MCF-7), as well as activated T-cells.
• Post-translational modifications and structural features: The protein contains a signal peptide, disulfide bonds, and is subject to glycosylation, phosphorylation, and methylation, as indicated by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 124–232 within the extracellular domain of human DR4; thus, the antibody likely recognizes epitopes in the ligand-binding region and may preferentially detect the native conformation.
• For flow cytometry (FC), staining of live, non-permeabilized cells is expected to label cell-surface DR4, while intracellular detection may require permeabilization.
• In ELISA applications, the antibody can be used for detection of recombinant or native DR4; sandwich immunoassays may benefit from pairing with a second antibody targeting a non-overlapping epitope.
• As a rabbit monoclonal antibody, it provides batch-to-batch consistency; optimal working concentrations should be determined empirically for each assay system.
• Cross-reactivity is reported for human DR4 only; performance with DR4 from other species has not been characterized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research institutes, and laboratories a streamlined, one-stop access to high-quality IVD raw materials. This anti-DR4 monoclonal antibody exemplifies our commitment to supplying validated reagents for apoptosis and oncology research. From optimized antibody pairs and bulk ancillary reagents to the sourcing of rare targets, CamelBio bridges the gap from concept to clinic—empowering your assay development with reliable, ready-to-use raw materials and expert technical support.