Anti-DPYSL3 Rabbit Polyclonal Antibody for WB, ELISA - Q14195

Core Product Specifications and Parameters

Biological Background: DPYSL3 Function and Localization

• Dihydropyrimidinase-related protein 3 (DPYSL3), also known as collapsin response mediator protein 4 (CRMP-4) or Unc-33-like phosphoprotein 1 (ULIP-1), belongs to the dihydropyrimidinase family and is encoded by the DPYSL3 gene.
• It is essential for semaphorin 3 signaling pathways that lead to cytoskeletal remodeling, playing a critical role in axon guidance, neuronal growth cone collapse, and cell migration.
• The protein is predominantly located in the cytoplasm and accumulates at the growth cone, a dynamic structure at the tip of developing axons.
• DPYSL3 is mainly expressed in heart and skeletal muscle, with strong expression also detected in fetal brain and spinal cord, suggesting key functions in both muscle and developing neural tissues.
• Multiple isoforms are produced by alternative splicing, contributing to functional diversity.
• The protein undergoes phosphorylation, a modification known to regulate its activity in semaphorin-mediated repulsion responses.
• High-resolution 3D structures are available, providing insights into its molecular architecture, and it has been identified through both direct protein sequencing and proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen spans a region near the C-terminus (amino acids 440-520) of human DPYSL3, ensuring specific recognition of the target.
• For Western blotting, lysates from tissues with high endogenous expression (e.g., heart, skeletal muscle, fetal brain) can serve as positive controls; cross-reactivity with mouse and rat allows flexibility in model systems.
• When developing an ELISA, consider pairing this polyclonal antibody with a matched monoclonal antibody to establish a reliable sandwich assay.
• As a polyclonal preparation, lot-to-lot consistency may vary; validate each new lot in your specific sample system and optimize dilutions empirically.
• The predicted molecular weight is approximately 62 kDa, but phosphorylated forms or splice variants may show slight shifts; always use appropriate loading controls and molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For studies on neuronal development, cytoskeleton dynamics, or semaphorin signaling, this anti-DPYSL3 polyclonal antibody offers a well-characterized tool. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to streamline your assay development and production workflows.