Anti-DPYS Polyclonal Antibody for WB, ELISA - Q14117

Core Product Specifications and Parameters

Biological Background: DPYS Function and Localization

• DPYS encodes dihydropyrimidinase (DHP), a key enzyme in the reductive pathway of pyrimidine catabolism.
• It catalyzes the reversible hydrolytic ring opening of 5,6-dihydrouracil to N-carbamyl-β-alanine and 5,6-dihydrothymine to N-carbamyl-aminoisobutyrate.
• Predominantly expressed in the liver and kidney, consistent with its role in nucleotide degradation and nitrogen excretion.
• DPYS is a zinc-dependent metallohydrolase and belongs to the cyclic amidohydrolase family.
• Phosphorylation and several disease-associated missense variants have been reported, linking DPYS to dihydropyrimidinuria.
• The protein has a molecular weight of approximately 57 kDa, as confirmed by SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 250–519), which is well-conserved across human, mouse, and rat; consistent with the observed cross-reactivity.
• For Western blot, a single band near 57 kDa is typically detected; consider using a positive control lysate from liver or kidney.
• In ELISA applications, pairing with a capture antibody targeting a different epitope may be suitable for sandwich assay development.
• Optimize blocking and antibody dilution conditions for your specific sample matrix; validate in knockout or siRNA-treated models if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to validated raw materials, including this anti-DPYS antibody. Whether you need bulk polyclonal or monoclonal antibodies, matched antibody pairs, or ancillary reagents, our one-stop supply platform supports your workflow from early-stage development to clinical applications. Inquire about custom antibody generation for rare targets or assay-specific optimization—our team bridges the gap between concept and clinic.