Anti-DPYD Rabbit Polyclonal Antibody for WB, ELISA - Q12882

Core Product Specifications and Parameters

Biological Background: DPYD Function and Localization

• DPYD (dihydropyrimidine dehydrogenase) is the initial and rate-limiting enzyme of the pyrimidine catabolic pathway, catalyzing the NADPH-dependent reduction of uracil and thymine to dihydrouracil and dihydrothymine, respectively.
  Related references: PMID:1512248
• It is also responsible for the metabolic inactivation of the chemotherapeutic prodrug 5-fluorouracil (5-FU), converting it to dihydrofluorouracil and thereby modulating drug efficacy and toxicity.
  Related references: PMID:1512248
• Alternative designations include dihydrothymine dehydrogenase, dihydrouracil dehydrogenase, DPD, and DHPDHASE.
• The enzyme is widely expressed, with the highest catalytic activity found in liver and peripheral blood mononuclear cells.
• DPYD localizes predominantly to the cytoplasm.
• The protein contains multiple cofactors essential for its oxidoreductase function, including 4Fe-4S iron-sulfur clusters, FAD, FMN, and NADP.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (residues 1–173) of human DPYD; therefore, the antibody may detect multiple splice variants. Validate reactivity in your target tissue or cell line.
• For Western blotting, a specific band near 111 kDa is anticipated. Use appropriate gel percentage (e.g., 8–10% SDS-PAGE) and optimize blocking/dilution conditions to minimize non-specific binding.
• Include positive controls such as human liver lysates or mouse tissues rich in DPYD activity to confirm assay performance.
• For ELISA applications, test direct binding or explore sandwich pair compatibility with a matched detection antibody if quantitative purposes are required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, empowering diagnostic manufacturers and research teams to accelerate pyrimidine metabolism and 5-FU pharmacogenomic projects. In addition to this DPYD polyclonal antibody, we offer validated monoclonal/polyclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and customized procurement for rare and niche targets—all designed to streamline your workflow from discovery to clinical application.