Anti-DPPA3/Stella Polyclonal Antibody for WB and ELISA - Q8QZY3

Core Product Specifications and Parameters

Biological Background: Stella Function and Localization

• DPPA3, also known as Stella, PGC7, or developmental pluripotency-associated protein 3, is a primordial germ cell (PGC)-specific protein crucial for early embryonic development.
• It plays a central role in epigenetic reprogramming at fertilization: specifically protects the maternal pronucleus and certain paternal imprinted loci from TET3-mediated oxidation of 5mC to 5hmC, thereby preserving DNA methylation patterns.
• Stella recognizes and binds histone H3 dimethylated at lysine 9 (H3K9me2) on the maternal genome; this mark is abundant in the maternal pronucleus but not in the paternal pronucleus, which is largely packaged with protamines.
• The protein is also involved in chromatin condensation during oocytogenesis and may contribute to maintaining the totipotent/pluripotent states during preimplantation development.
• Subcellularly, DPPA3 localizes to both the nucleus and the cytoplasm, consistent with its roles in chromatin interaction and cytoplasmic processes.
• Tissue expression is restricted to germ cells and early embryos: detected in immature oocytes, maturing oocytes, preimplantation embryos, blastocysts, epiblasts, and primordial germ cells; expression is lost in differentiated somatic cells and adult testes.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein encompassing amino acids 1–150 of human Stella, suggesting the antibody may target epitopes within this N-terminal region. Cross-reactivity with mouse and rat implies conserved sequences; validate specificity in your species of interest.
• For Western blotting, an expected band at approximately 18 kDa should be detected in samples from mouse oocytes, early embryos, or germ cell lines. Due to restricted expression, careful attention to sample preparation and loading controls is recommended.
• In ELISA, consider using the antibody for detection or capture in assays requiring recognition of Stella. Optimal working concentrations should be determined experimentally for each application.
• As with any polyclonal antibody, lot-to-lot variation may occur; inclusion of appropriate positive and negative controls (e.g., Stella-knockout tissues) can enhance confidence in results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including this DPP3A/Stella polyclonal antibody. As a B2B sourcing partner, we offer tailored solutions such as validated antibody pairs, bulk monoclonal and polyclonal antibodies, and essential ancillary reagents, streamlining your workflow from concept to clinic. Whether your focus is developmental biology or epigenetic markers, our team can assist in sourcing even the rarest targets to meet your project needs.