Anti-DPPA2 Polyclonal Antibody for WB, ELISA - Q7Z7J5

Core Product Specifications and Parameters

Biological Background: DPPA2 Function and Localization

• DPPA2 (Developmental pluripotency-associated protein 2), also known as pluripotent embryonic stem cell-related gene 1 protein (PESCRG1) or CT100/ECAT15-2, is a nuclear protein involved in transcription regulation.
• It binds to target gene promoters such as NKX2-5 and SYCE1, potentially maintaining their active epigenetic status, but does not bind to GATA4, indicating promoter specificity.
• The protein functions as a transcriptional regulator associated with the maintenance of pluripotency and early embryonic development.
• Subcellular localization is exclusively nuclear, consistent with its role in gene regulation.
• DPPA2 is expressed specifically in embryonic stem cells and is absent in later developmental stages (e.g., 5-month embryo), mesenchymal stem cells, embryonic fibroblasts, and adult tissues.
• Its molecular weight is approximately 33.8 kDa (calculated), matching the observed ~34 kDa band in western blotting.
• DPPA2 is catalogued under keywords related to nucleus, proteomics identification, reference proteome, and transcription regulation, underscoring its role in chromatin-associated processes.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-200 of human DPPA2) covers the N-terminal region, which is likely exposed and accessible for antibody binding; consider this when designing epitope-mapping or competitive assays.
• DPPA2 is predominantly nuclear; ensure cell fractionation methods are optimized for nuclear protein extraction in your sample preparation for WB.
• The antibody is validated for WB and ELISA; for WB, a band at ~34 kDa is expected; titrate antibody concentration to optimize signal-to-noise ratio, especially in stem cell lysates which may have low target abundance.
• Cross-reactivity with mouse and rat is predicted based on immunogen sequence homology; however, it is advisable to validate reactivity and specificity in the intended species before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. The Anti-DPPA2 polyclonal antibody is suitable for researchers exploring pluripotency and stem cell biology, and CamelBio can provide matched antibody pairs, optimized clones, and bulk supply to accelerate your assay development. Whether you need rare-target antibodies or routine reagents, our integrated supply chain ensures reliable access for your workflow.