Anti-DPP8 Polyclonal Antibody for WB and ELISA - Q6V1X1

Core Product Specifications and Parameters

Biological Background: DPP8 Function and Localization

• DPP8 is a dipeptidyl peptidase that cleaves N-terminal dipeptides from proteins with Pro or Ala at position 2. Related references: PMID:11012666 PMID:12534281 PMID:12662155
• Acts as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis by preventing activation of NLRP1 and CARD8 inflammasomes. Related references: PMID:27820798 PMID:29967349 PMID:32796818
• Sequesters the cleaved C-terminal active part of NLRP1 and CARD8 in a ternary complex, thereby preventing their oligomerization and activation. Related references: PMID:33731929 PMID:33731932 PMID:34019797
• DPP8 belongs to the serine protease family and is classified as a prolyl dipeptidase.
• Localizes predominantly to the cytoplasm.
• Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle, and brain.
• Keywords indicate the presence of alternative splicing isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 10-230 of human DPP8 (N-terminal region). For Western blot, expect a band near 103 kDa. Consider using a loading control appropriate for your sample.
• For ELISA, this antibody may be suitable for both direct and capture formats; validate with recombinant or native protein standards.
• The reported cross-reactivity with mouse and rat supports use in comparative studies across these species; confirm species-specific detection in your experimental setup.
• As with any polyclonal antibody, performance may vary between lots; it is advisable to test each new batch in your established assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research laboratories with streamlined access to a comprehensive portfolio of raw materials, including validated antibodies, antigens, and ancillary reagents. For targets like DPP8 involved in inflammasome regulation and pyroptosis, we offer polyclonal and monoclonal antibodies suitable for diagnostic assay development. Our supply chain expertise enables reliable sourcing of rare specificities and bulk quantities, supporting your projects from biomarker discovery to clinical application.