Anti-DPP3 Rabbit Monoclonal Antibody for WB and ELISA - Q9NY33

Core Product Specifications and Parameters

Biological Background: DPP3 Function and Localization

• DPP3 (Dipeptidyl peptidase 3), also known as DPP III, dipeptidyl aminopeptidase III, or enkephalinase B, is a zinc-dependent metalloprotease that cleaves dipeptides from the N-terminus of bioactive peptides, including angiotensin, Leu-enkephalin, and Met-enkephalin.
  Related references:
  PMID:1515063
  PMID:3233187
• Exhibits in vitro cleaving activity towards Arg-Arg-beta-naphthylamide.
  Related references:
  PMID:11209758
  PMID:3233187
  PMID:9425109
• Localizes predominantly to the cytoplasm and cytosol.
• Detected at the protein level in placenta and erythrocytes.
  Related references:
  PMID:1515063
  PMID:3233187
• Alternative splicing generates multiple isoforms (UniProt keyword: Alternative splicing).
• Subject to post-translational acetylation (UniProt keyword: Acetylation).
• Contains a zinc-binding motif essential for catalytic activity (UniProt keywords: Metal-binding, Zinc).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 638-737) of human DPP3, suggesting the antibody may target a linear epitope; consider this when designing antigen retrieval or denaturing conditions for WB.
• For Western blot, the predicted molecular weight is approximately 83 kDa for human DPP3; validate band size using positive controls from human, mouse, or rat lysates, as actual migration can shift due to modifications.
• In ELISA, the synthetic immunogen peptide can serve as a positive control to confirm binding specificity.
• Reported cross-reactivity with mouse and rat implies sequence homology in the immunogen region; verify expected reactivity by aligning target sequences if using other species.
• Store at -20°C (shipped on ice bags) and minimize freeze-thaw cycles to maintain antibody stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement with validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing—supporting your research on DPP3 and bioactive peptide degradation. As a comprehensive partner, we help diagnostic manufacturers and labs accelerate development from concept to clinic.