Anti-DPM1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O60762

Core Product Specifications and Parameters

Biological Background: DPM1 Function and Localization

• DPM1 encodes the catalytic subunit of the dolichol-phosphate mannose (DPM) synthase complex, which transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man).
• Dol-P-Man is a critical mannosyl donor required for N-glycosylation, glycosylphosphatidylinositol (GPI) anchor biosynthesis, and O-mannosylation of proteins.
• The protein is localized to the endoplasmic reticulum membrane, consistent with its role in the early secretory pathway.
• DPM1 undergoes post-translational modifications including acetylation and phosphorylation, and requires magnesium or manganese ions for its transferase activity.
• Mutations in DPM1 are associated with congenital disorders of glycosylation (CDG) type Ie and congenital muscular dystrophy with dystroglycanopathy, highlighting its importance in development.

Experimental Guidance and Technical Tips

• Given the full-length recombinant immunogen, this polyclonal antibody likely detects multiple epitopes, making it suitable for both native and denatured conditions in applications like WB, IF/ICC, and ELISA.
• For Western blot, expect a band at approximately 30 kDa corresponding to DPM1; optimize sample preparation and loading controls for your specific cell or tissue lysate.
• In immunofluorescence/ICC, permeabilization is required to access the cytoplasmic/ER-localized DPM1 protein; consider co-staining with ER markers to confirm subcellular distribution.
• For ELISA, titrate the antibody against recombinant DPM1 or cell lysates to establish optimal coating and detection conditions; validate specificity with appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting to support your diagnostic and research programs from concept to clinic. This DPM1 rabbit polyclonal antibody is tailored for glycosylation-related research and assay development, backed by our expertise in supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Leverage our global sourcing capabilities for even the most niche targets, ensuring your projects stay on track with reliable, high-quality materials.