Anti-DPH2 Polyclonal Antibody for WB, ELISA - Q9BQC3

Core Product Specifications and Parameters

Biological Background: DPH2 Function and Localization

• DPH2 encodes subunit 2 of 2-(3-amino-3-carboxypropyl)histidine synthase, also known as diphthamide biosynthesis protein 2, essential for the unique post-translational modification of elongation factor 2.
• Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. Related references: PMID:32576952
• Together with DPH1, transfers a 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine to the target histidine; the reaction depends on the DPH3 and NADH-dependent reductase system.
• Facilitates reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit, underlining its role in enzyme activation.
• Strongly expressed in skeletal muscle; moderately in heart, small intestine, liver, pancreas, testis, and colon; weakly in brain, placenta, kidney, spleen, thymus, prostate, ovary, and lymphocytes.
• Post-translational modifications include acetylation and phosphorylation; iron-sulfur cluster binding is critical for function.
• Mutations in DPH2 are associated with developmental disorders featuring dwarfism, ectodermal dysplasia, hypotrichosis, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 120–330, a region that may be surface-exposed and is conserved between human and mouse, supporting the observed cross-reactivity.
• For Western blotting and ELISA, titrate the antibody and validate in your sample matrix; positive signals are expected around 52 kDa.
• Include positive controls from tissues with high DPH2 expression, such as skeletal muscle or heart, and consider cross-reactivity when using mouse models.
• Verify sequence alignment if applying to other species, and optimize detection conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-DPH2 antibody is part of our growing portfolio for diphthamide biosynthesis and translational elongation research, backed by reliable supply channels. Partner with us to bridge the gap from concept to clinic with one-stop access to critical immunoassay components.