Anti-DPF3 Rabbit Polyclonal Antibody for WB, ELISA - Q92784

Core Product Specifications and Parameters

Biological Background: DPF3 Function and Localization

• DPF3, also known as BAF45C or CERD4, is a zinc finger protein that acts as a tissue-specific subunit of the mammalian SWI/SNF (BAF) chromatin remodeling complex.
• As part of the neuron-specific nBAF complex, DPF3 replaces PHF10/BAF45A during neural differentiation, facilitating the transition from neural stem/progenitor cells to post-mitotic neurons and regulating dendrite growth (By similarity).
• In muscle, DPF3 anchors the BAF complex to acetylated histones (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac) bridging histone acetylation and methylation marks to drive chromatin remodeling for heart and skeletal muscle development.
• DPF3 cooperates with HDGFL2 to regulate myogenesis by recruiting SMARCA4/BRG1 to myogenic gene promoters, increasing chromatin accessibility and activating myogenic genes. Related references: PMID:32459350
• The protein is localized exclusively in the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include phosphorylation and ubiquitination, as indicated by proteomics data, and the protein contains multiple zinc-finger domains (PHD-type) that mediate histone binding.
• DPF3 belongs to the DPF family of BAF45 subunits, which are critical for lineage-specific chromatin remodeling during neurogenesis and myogenesis.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering the N-terminal region (aa 90-200) of human DPF3. This region may contain conserved domains; cross-reactivity with mouse and rat DPF3 has been observed.
• For western blotting, the predicted molecular weight is approximately 43 kDa; consider verifying band specificity using appropriate controls (e.g., knockdown or knockout lysates).
• For ELISA, consider optimizing coating antigen and antibody dilution based on the specific plate format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to validated IVD raw materials, including rabbit polyclonal antibodies like anti-DPF3 for chromatin biology and neurogenesis research. Our supply capabilities encompass optimized antibody pairs, bulk ancillary reagents, and sourcing support for rare targets, ensuring a seamless procurement experience from concept to clinic.