Anti-DPF2 Rabbit Polyclonal Antibody for WB and ELISA - Q92785

Core Product Specifications and Parameters

Biological Background: DPF2 Function and Localization

• DPF2 (zinc finger protein ubi-d4, also known as BAF45D or Protein requiem) is a member of the d4 family and contains a double PHD finger domain that binds to modified histones H3 and H4, enabling it to regulate chromatin structure and transcription. Related references: PMID:27775714 PMID:28533407
• It functions as a negative regulator of myeloid differentiation in hematopoietic progenitor cells. Related references: PMID:28533407
• DPF2 is involved in the regulation of the non-canonical NF-kappa-B signaling pathway, which plays a role in immune and inflammatory responses. Related references: PMID:20460684
• The protein may also contribute to the development and maturation of lymphoid cells (By similarity).
• DPF2 localizes to both the nucleus and the cytoplasm, consistent with its dual roles in transcriptional regulation and signaling.
• It is ubiquitously expressed across human tissues, reflecting its fundamental role in chromatin biology.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitination, are predicted to modulate DPF2 activity and protein interactions.
• Mutations in the DPF2 gene have been associated with intellectual disability, highlighting its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the N-terminal portion (aa 90-200) of human DPF2, which may encompass part of the PHD finger domain; consider that the antibody could recognize structured epitopes.
• For Western blotting, use whole-cell lysates from human, mouse, or rat cells, as the antibody shows cross-reactivity with these species.
• The predicted molecular weight of DPF2 is approximately 44 kDa; select gel and transfer conditions appropriate for proteins in this size range.
• For ELISA applications, both direct antigen coating and sandwich formats may be considered, with optimization of coating and detection concentrations based on the experimental system.
• Ubiquitous expression of DPF2 suggests that common cell lines (e.g., HeLa, HEK293, Jurkat) can serve as positive controls; however, expression levels should be verified in the specific context.
• Although validated for WB and ELISA, the antibody’s reactivity against native protein suggests potential suitability for applications such as immunoprecipitation (IP) or chromatin immunoprecipitation (ChIP), but independent validation is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw-material supply, offering this DPF2 polyclonal antibody alongside a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise extends to rare target sourcing and technical consultation, supporting research in chromatin biology, transcriptional regulation, and beyond. From early-stage development to clinical application, CamelBio streamlines your procurement process, ensuring a reliable supply chain for diagnostic manufacturers and research laboratories alike.