Anti-DPEP2 Rabbit Polyclonal Antibody for WB, ELISA - Q9H4A9

Core Product Specifications and Parameters

Biological Background: DPEP2 Function and Localization

• DPEP2 (Dipeptidase 2), also known as MBD2, is a membrane-bound dipeptidase that belongs to the metalloprotease family.
• Functions as a hydrolase that catalyzes the conversion of leukotriene D4 (LTD4) to leukotriene E4 (LTE4), playing a role in lipid metabolism and inflammatory signaling (PubMed:32325220). Related references: PMID:32325220
• Also hydrolyzes cystinyl-bis-glycine, indicating broader substrate specificity in peptide cleavage (PubMed:32325220). Related references: PMID:32325220
• Beyond its enzymatic activity, DPEP2 can modulate macrophage inflammatory responses by acting as a regulator of the NF-kappaB signaling pathway.
• Localized to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor, with a signal peptide directing its membrane insertion.
• Post-translational modifications include glycosylation and the formation of disulfide bonds essential for structural integrity.
• Requires zinc ion binding as a metal-dependent protease (metalloprotease), with the zinc-binding motif critical for catalytic activity.
• Alternative splicing generates multiple transcript variants, potentially producing isoforms with distinct functions or localizations.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 400-463), so the antibody may detect full-length DPEP2 as well as any cleavage products or alternatively spliced isoforms containing this epitope.
• For Western blot (WB), a specific band around 53 kDa is anticipated in human, mouse, and rat samples; optimize loading controls and blocking conditions accordingly.
• For ELISA applications, consider using recombinant DPEP2 protein or cell lysates from relevant tissues; validate with appropriate coating buffers and detection reagents.
• Given the GPI-anchored membrane localization, inclusion of non-ionic detergents in lysis buffers is recommended to enhance protein solubility for immunodetection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers and research laboratories from initial development to clinical application. For DPEP2-related studies in inflammation and lipid metabolism, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored to your workflow. Our dedicated raw-material sourcing capabilities help simplify procurement, allowing you to focus on advancing your diagnostic or research objectives.