Anti-DPEP1 Rabbit Polyclonal Antibody for WB, ELISA - P16444

Core Product Specifications and Parameters

Biological Background: DPEP1 Function and Localization

• DPEP1 (Dipeptidase 1), also known as MDP, RDP, MBD1, is a zinc metalloprotease and a member of the dipeptidase family.
• It hydrolyzes a broad range of dipeptides, including the conversion of leukotriene D4 to leukotriene E4 and the cleavage of cystinyl-bis-glycine during glutathione degradation. Related references: PMID:2303490, PMID:31442408, PMID:32325220
• Exhibits beta-lactamase activity, capable of hydrolyzing the beta-lactam antibiotic imipenem. Related references: PMID:32325220, PMID:6334084
• Acts as an adhesion receptor for neutrophil recruitment into inflamed lungs and liver, independent of its enzymatic activity.
• Localized to the apical cell membrane and microvillus membrane via a GPI-anchor.
• Expressed in lung and kidneys.
• Post-translational modifications include glycosylation, GPI-anchor, and disulfide bond formation; contains a signal peptide.

Experimental Guidance and Technical Tips

• Confirm antibody specificity by Western blot using lysates from DPEP1-expressing tissues (lung, kidney) or cell lines; a band near 46 kDa is expected under reducing conditions.
• For ELISA, the recombinant immunogen (aa 17-385) can serve as a positive control to assess binding.
• The antibody detects both human and mouse DPEP1 as per supplier's cross-reactivity data; validate cross-reactivity in your experimental system.
• Optimal working dilutions for WB and ELISA should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing experience for IVD raw materials, from concept to clinic. For researchers targeting DPEP1 in leukotriene metabolism or adhesion, we supply high-quality antibodies alongside complementary reagents like recombinant proteins and ELISA kits. Reach out for volume orders or technical consultation.