Anti-DP1/TFDP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14186

Core Product Specifications and Parameters

Biological Background: DP1/TFDP1 Function and Localization

• DP1, also known as Transcription factor Dp-1, is the protein product of the TFDP1 gene and serves as a critical dimerization partner for E2F transcription factors.
• It stimulates E2F-dependent transcription by binding cooperatively with E2F family members to the E2 recognition site (5'-TTTC[CG]CGC-3') found in promoters of genes involved in cell cycle regulation and DNA replication. Related references: PMID:7739537, PMID:8405995
• The E2F1:DP complex mediates both cell proliferation and apoptosis, and DP1 blocks adipocyte differentiation by repressing CEBPA binding to target gene promoters. Related references: PMID:20176812
• Subcellular localization is primarily nuclear, but the protein is also detected in the cytoplasm, suggesting dynamic shuttling between these compartments.
• Expression is highest in muscle, with moderate levels in brain, placenta, liver, and kidney, low levels in lung and pancreas, and no detection in heart.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; alternative splicing generates multiple isoforms, as highlighted by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 311–410 near the C-terminus; the antibody is expected to detect full-length DP1, but isoforms missing this epitope may not be recognized. Validate in your sample system.
• For Western blotting, a band around 45 kDa is anticipated. Include appropriate positive controls (e.g., HeLa or HEK293 lysates) and loading controls.
• In IHC-P, optimize antigen retrieval and consider that nuclear staining is predominant, though cytoplasmic signal may also appear due to shuttling.
• This antibody shows cross-reactivity with human and rat; for other species, verify target sequence homology before use.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For cell cycle and transcriptional regulation research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to streamline your assay development and manufacturing needs.