Anti-DP1/TFDP1 Polyclonal Antibody for WB, IHC-P, ELISA - Q14186

Core Product Specifications and Parameters

Biological Background: DP1/TFDP1 Function and Localization

• Transcription factor Dp-1 (TFDP1) is a key component of the E2F transcription factor complex, serving as the obligate dimerization partner for E2F family proteins.

• DP1 binds DNA cooperatively with E2F through the E2 recognition site 5'-TTTC[CG]CGC-3' found in promoters of genes involved in cell cycle progression and DNA replication.

  Related references: PMID:7739537, PMID:8405995

• The E2F1:DP complex appears to mediate both cell proliferation and apoptosis, highlighting its dual role in cell fate decisions.

• DP1 blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters.

  Related references: PMID:20176812

• DP1 localizes to both the nucleus and cytoplasm, enabling its transcriptional regulatory functions and potential cytoplasmic roles.

• Tissue expression is highest in muscle, with moderate levels in brain, placenta, liver, and kidney; lower in lung and pancreas; and undetectable in heart.

• DP1 undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which modulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-290), so the antibody is expected to recognize full-length DP1 (~45 kDa). Validate detection in relevant cell or tissue lysates.
• For Western blotting, optimize antibody dilution; a band around 45 kDa is anticipated. Use appropriate loading controls.
• For immunohistochemistry (paraffin), standard antigen retrieval methods are recommended; titrate antibody for optimal signal-to-noise ratio.
• This antibody cross-reacts with mouse, making it suitable for comparative studies. For other species, perform sequence alignment and test experimentally.
• In ELISA applications, consider using a validated antigen as positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For the DP1/TFDP1 target and cell cycle research, we supply optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare target sourcing, ensuring a seamless procurement experience for your assay development needs.