Anti-Doublecortin Rabbit Monoclonal Antibody for WB, ELISA - O43602

Core Product Specifications and Parameters

Biological Background: Doublecortin Function and Localization

• Doublecortin (DCX), also known as Doublin, Lis-X, or LISX, is a microtubule-associated protein essential for neuronal migration and cortex lamination during brain development.
• It functions in the initial steps of neuronal dispersion and may compete with the protein kinase DCLK1 for target binding, potentially participating in a calcium-dependent signaling pathway.
• DCX cooperates with PAFAH1B1/LIS-1 in overlapping but distinct pathways that promote proper neuronal migration.
• The protein localizes to the cytoplasm and neuron projections, consistent with its role in neuronal migration.
• DCX is highly expressed in fetal brain regions (cortical plate, intermediate and ventricular zones) and in the adult frontal lobe, with low or absent expression in other tissues.
• Post-translational modifications include phosphorylation and ubiquitination (UBL conjugation), which may regulate its function and stability.
• Genetic variants in DCX are associated with developmental disorders such as lissencephaly and epilepsy, often involving chromosomal rearrangements.

Experimental Guidance and Technical Tips

• Based on the immunogen design encompassing amino acids 265–365, the antibody likely recognizes an epitope within the C-terminal region of human Doublecortin; users should consider epitope conservation when applying to mouse or rat samples.
• For Western blot analysis, it is advisable to use protein extracts from fetal or adult brain tissues where DCX expression is highest, and to validate detection around the predicted 41 kDa band.
• In ELISA applications, consider using a recombinant Doublecortin protein standard to optimize assay sensitivity and specificity.
• Since this is a recombinant rabbit monoclonal antibody, lot-to-lot consistency can be expected; nevertheless, each new lot should be verified in the relevant experimental system.
• To assess cross-reactivity with other DCX family members or isoforms, sequence alignment and pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. To support your Doublecortin-related neurodevelopment and lissencephaly research, CamelBio can provide custom antibody panel design, bulk production, and rare target sourcing, streamlining your transition from concept to clinic.