Anti-Doublecortin Rabbit Monoclonal Antibody for WB, ELISA - O43602

Core Product Specifications and Parameters

Biological Background: Doublecortin Function and Localization

• Doublecortin (DCX) is a microtubule-associated protein essential for neuronal dispersion and cortex lamination during cerebral cortex development. It likely acts by competing with DCLK1 kinase in binding to a target protein, potentially participating in a calcium-dependent signal transduction pathway crucial for neuronal migration.
• DCX is part of overlapping but distinct signaling pathways with PAFAH1B1/LIS-1, both promoting neuronal migration.
• Also known as Doublin and Lissencephalin-X, mutations in DCX are associated with lissencephaly and epilepsy.
• The protein localizes to the cytoplasm and neuron projections.
• It is highly expressed in neuronal cells of the fetal brain, particularly in the cortical plate, intermediate zone, and ventricular zone. In adults, expression is prominent in the frontal lobe of the brain but very low elsewhere, and absent from non-neuronal tissues such as heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• DCX undergoes phosphorylation and ubiquitination (Ubl conjugation), and alternative splicing generates multiple isoforms. It contains repeat domains and is classified as a developmental protein involved in neurogenesis and differentiation.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band of approximately 41 kDa is expected. Consider using lysates from neuronal tissues or cell lines (e.g., fetal brain or adult frontal lobe) as positive controls.
• The antibody is validated for ELISA; consider using the immunogen peptide or recombinant DCX protein for assay development.
• Cross-reactivity with mouse and rat allows for studies in common rodent models of neurodevelopment. Validate the antibody in the relevant sample system before large-scale experiments.
• As a monoclonal antibody, it offers consistent lot-to-lot performance, suitable for reproducible quantitative assays.

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CamelBio supports diagnostic manufacturers, research laboratories, and institutes with a comprehensive portfolio of IVD raw materials. For neuroscience and developmental biology applications, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our team also assists in sourcing rare-target raw materials, helping you streamline your assay development from concept to clinic.