Anti-Doublecortin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - O43602

Core Product Specifications and Parameters

Biological Background: Doublecortin Function and Localization

• Doublecortin (DCX), also known as Doublin or Lis-X, is a microtubule-associated protein encoded by the DCX gene; alternative splicing produces multiple isoforms.
• It plays a critical role in neuronal dispersion and cortex lamination during cerebral cortex development, acting by competing with the neuronal protein kinase DCLK1 for target binding and participating in a calcium ion-dependent signaling pathway.
• DCX localizes to the cytoplasm and is associated with neuron projections, consistent with its function in neuronal migration.
• The protein is highly expressed in neuronal cells of the fetal brain (cortical plate, intermediate zone, and ventricular zone) but not in other fetal tissues; in adults, it is highly expressed in the brain frontal lobe, with very low expression in other brain regions and undetectable in peripheral tissues.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation, which may regulate its activity and stability.
• Mutations or chromosomal rearrangements involving DCX are associated with lissencephaly and epilepsy, highlighting its essential role in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 265-365 of human Doublecortin; consider using a peptide blocking experiment to confirm antibody specificity in your sample type.
• For Western blotting, a band at approximately 41 kDa is expected; validate detection using appropriate positive and negative controls.
• In immunofluorescence applications, optimize fixation and permeabilization conditions to preserve microtubule-associated staining in neuronal cells.
• Given cross-reactivity with mouse and rat, this antibody is suitable for studies in rodent models of neurodevelopment.
• For ELISA, titrate the antibody and consider pairing with a matched detector antibody if used in sandwich format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutions with one-stop access to IVD raw materials, technical services, and consulting. This anti-Doublecortin antibody is part of our extensive catalog for neurodevelopmental research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your development pipeline.