Anti-Doublecortin Polyclonal Antibody for WB, IF/ICC, ELISA - O43602

Core Product Specifications and Parameters

Biological Background: Doublecortin Function and Localization

• DCX encodes Neuronal migration protein doublecortin (also known as Doublin, Lissencephalin-X), a microtubule-associated protein essential for neuronal migration during cerebral cortex development.
• It plays a key role in the initial steps of neuronal dispersion and cortex lamination, possibly by competing with DCLK1 for target protein binding within a calcium-dependent signaling pathway.
• The protein contains two conserved doublecortin (DCX) domains that mediate binding to and stabilization of microtubules, critical for neuronal migration.
• Subcellular localization is predominantly cytoplasmic and enriched in neuron projections, consistent with its function in migrating neurons.
• Tissue expression is highly specific: in fetal brain, DCX is abundant in cells of the cortical plate, intermediate zone, and ventricular zone; in adults, it is highly expressed in the frontal lobe but at very low levels in other brain regions and absent from non-neural tissues.
• Post-translational modifications include phosphorylation and ubiquitination, which may regulate its activity and turnover.
• Mutations in DCX cause X-linked lissencephaly and subcortical band heterotopia, underscoring its critical role in neurogenesis and proper brain development.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider a band at approximately 41 kDa; validation in neuronal tissue lysates (e.g., fetal brain or adult frontal lobe) is recommended.
• In immunofluorescence/ICC experiments, signal is expected in the cytoplasm and along neuron projections; appropriate fixation and permeabilization protocols should be optimized.
• This polyclonal antibody is generated against amino acids 265–365, a region likely spanning part of the C-terminal domain; epitope-specific performance may vary by application.
• Cross-reactivity with mouse and rat is indicated; however, independent validation in the intended model system is suggested to confirm specificity.
• For ELISA, use recombinant protein or cell lysates as antigen sources; titrate antibody and detection conditions for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials and technical support. This anti-Doublecortin polyclonal antibody supports neuronal development and migration research, and we can assist in sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials to accelerate your project from concept to clinical application.