Anti-Dopamine Receptor D3 (DRD3) Rabbit Monoclonal Antibody for WB, ELISA - P35462

Core Product Specifications and Parameters

Biological Background: Dopamine Receptor D3 Function and Localization

• D(3) dopamine receptor (DRD3/D3DR) is a G protein-coupled receptor encoded by the DRD3 gene, with alternative splicing variants documented.
• Functions as a dopamine receptor primarily in limbic brain areas, modulating cognitive, emotional, and endocrine functions, and plays a key role in motivation, reward, and behavior. Couples to G(i)/G(o) proteins to inhibit adenylate cyclase and reduce intracellular cAMP levels.
  Related references:
  PMID:39984436 PMID:10578130
• Promotes cell proliferation via MAP kinase signaling.
  Related references:
  PMID:19520868
• Positively regulates autophagy: activation stimulates AMPK, which phosphorylates RPTOR, enhancing MTOR interaction and inhibiting MTORC1, leading to ULK1 activation and autophagy initiation.
  Related references:
  PMID:31538542
• Forms heterotetramers with DRD1 to enhance beta-arrestin recruitment and regulate locomotor activity.
• Subcellularly localizes to the cell membrane.
• Shows brain-specific tissue expression.
• Contains structural features typical of GPCRs, including seven transmembrane helices, disulfide bonds, glycosylation, and palmitoylation sites.
• Associated with neuropsychiatric conditions such as schizophrenia and substance use disorders.
  Related references:
  PMID:39984436

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), enabling detection of total DRD3 protein; consider this when selecting lysates and blocking conditions.
• For WB, the predicted molecular weight is ~44 kDa; validate specificity using brain tissue lysates from human, mouse, or rat, as the antibody cross-reacts with these species.
• For ELISA, consider using the immunogenic peptide or recombinant DRD3 protein as coating antigen; optimize coating concentration and antibody dilution experimentally.
• Because DRD3 undergoes glycosylation and palmitoylation, the observed MW on SDS-PAGE may differ from the predicted 44 kDa; consider using deglycosylation enzymes if band shifts are observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing channel for high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom procurement for rare targets. This anti-DRD3 rabbit monoclonal antibody supports neuroscience and neuropsychiatric research applications such as WB and ELISA. Our integrated supply capabilities help expedite your assay development from concept to clinic.