Anti-Dopamine Receptor D3 (DRD3) Polyclonal Antibody for WB, ELISA - P35462

Core Product Specifications and Parameters

Biological Background: Dopamine Receptor D3 (DRD3) Function and Localization

• Dopamine receptor D3 (DRD3), also known as D3DR, ETM1, or FET1, is a G protein-coupled receptor encoded by the DRD3 gene; alternative splicing produces multiple isoforms.
• It is primarily expressed in limbic brain regions and modulates cognitive, emotional, and endocrine functions, as well as neuronal signaling linked to motivation, reward, and behavior. Related references: PMID:39984436
• Couples to G(i)/G(o) proteins, inhibiting adenylate cyclase and decreasing intracellular cAMP levels. Related references: PMID:10578130
• Implicated in the control of locomotor activity and associated with neuropsychiatric disorders including schizophrenia and substance use disorders. Related references: PMID:39984436
• Promotes cell proliferation through MAP kinase signaling. Related references: PMID:19520868
• Regulates autophagy by stimulating AMPK, leading to RPTOR phosphorylation and MTORC1 inhibition, which activates ULK1 and initiates autophagy. Related references: PMID:31538542
• Forms heterotetramers with DRD1 to potentiate beta-arrestin recruitment and mediate locomotor activity.
• Localized to the cell membrane, the receptor undergoes post-translational modifications including disulfide bond formation, glycosylation, and palmitoylation.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is 44 kDa; validate band size using lysates from brain tissue or DRD3-expressing cell lines. Cross-reactivity with mouse and rat has been observed, but confirm in the relevant species.
• For ELISA, consider using the immunizing peptide as a competitive inhibitor to assess antibody specificity.
• As a polyclonal antibody, lot-to-lot variability is possible; include positive and negative controls in each experiment.
• Pre-adsorption with the immunogen peptide may be used to confirm specific binding in immunochemical applications.
• The immunogen targets the N-terminal region (amino acids 1-100) of human DRD3; sequence alignment suggests conservation across mouse and rat, supporting cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This polyclonal antibody against Dopamine Receptor D3 supports neuroscience research and IVD assay development for neuropsychiatric disorders. We offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring reliable supply for your diagnostic programs.