Anti-DOK5 Polyclonal Antibody for WB, ELISA - Q9P104

Core Product Specifications and Parameters

Biological Background: DOK5 Function and Localization

• Docking protein 5 (DOK5), also known as insulin receptor substrate 6 (IRS-6), is encoded by the DOK5 gene (C20orf180) in humans.
• DOK5 is a member of the DOK family of adaptor/scaffolding proteins that provide docking platforms for assembly of multimolecular signaling complexes.
• It functions as a positive regulator of RET-mediated neurite outgrowth and activates the MAP kinase pathway, playing a role in neuronal differentiation.
• DOK5 is an enzymatically inert phosphoprotein, exerting its effects through protein–protein interactions rather than catalytic activity.
• Highest expression is observed in skeletal muscle, with lower levels in brain, heart, kidney, and activated peripheral blood T-lymphocytes.
• The protein undergoes alternative splicing and has a known three-dimensional structure; it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen used covers amino acids 237-306, a region within the C-terminal portion of human DOK5. When planning detection strategies, consider epitope accessibility in your sample type.
• For Western blotting, test lysates from tissues or cells with known DOK5 expression (e.g., skeletal muscle, brain) and optimize loading amounts and antibody dilution to achieve clear bands at the predicted molecular weight (~35 kDa).
• Given the reported cross-reactivity with mouse and rat, validate specificity in your model system using appropriate controls, as sequence homology may influence reactivity.
• For ELISA applications, pair with a suitable detection antibody and use recombinant DOK5 or peptide as a standard to assess sensitivity and dynamic range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like DOK5 involved in RET signaling and neuronal differentiation, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Streamline your procurement with a partner committed to quality and comprehensive supply solutions.