Anti-DOK4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8TEW6

Core Product Specifications and Parameters

Biological Background: DOK4 Function and Localization

• DOK4 is a member of the DOK family of adaptor/scaffold proteins that provide docking platforms for the assembly of multimolecular signaling complexes.
• It functions downstream of receptor tyrosine kinases, including RET, and is implicated in RET-mediated neurite outgrowth and neuronal differentiation.
• DOK4 positively regulates the MAP kinase pathway, linking RET activation to cellular growth and differentiation signals (By similarity).
• The protein is also known as insulin receptor substrate 5 (IRS5) and may participate in insulin and immune signaling pathways.
• DOK4 is widely expressed, with highest levels in skeletal muscle, heart, kidney, and liver; moderate expression in spleen, lung, small intestine, and brain.
• It is a phosphoprotein, indicating regulation by post-translational modifications, and serves as a reference proteome component for human studies.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-326), making it suitable for detecting full-length DOK4 in WB; consider using positive controls like skeletal muscle or heart lysates to confirm specificity at ~37 kDa.
• For IHC-P and IF/ICC, standard antigen retrieval methods may be employed; validate antibody reactivity in human, mouse, or rat tissues as per cross-reactivity data.
• When performing ELISA, consider appropriate coating conditions and detection system; pair with a validated DOK4-specific detection antibody if quantitation is required.
• Given DOK4's role in signaling pathways, investigate its activation state under various stimuli, and note that functional studies may require additional phospho-specific reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. For DOK4-targeted research, we support sourcing needs in cell signaling and neurological disease areas, ensuring reliable access to high-quality materials from early R&D to clinical application. Our global supply network assists manufacturers in acquiring rare-target raw materials, with a focus on consistent lot-to-lot performance and technical consultation.