Anti-DOK1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q99704

Core Product Specifications and Parameters

Biological Background: DOK1 Function and Localization

• DOK1 (docking protein 1), also known as p62(dok) or pp62, is a member of the DOK family of adaptor/scaffold proteins that serve as docking platforms for signaling complex assembly.
• It is characterized as an enzymatically inert adaptor, providing a platform for protein-protein interactions in signal transduction pathways.
• DOK1 appears to function as a negative regulator of the insulin signaling pathway, modulating metabolic responses downstream of the insulin receptor.
• It also plays a role in integrin activation by competing with talin for binding to ITGB3, thereby influencing cell adhesion and migration.
• The protein localizes to the cytoplasm and nucleus, with enrichment in the perinuclear region, suggesting dynamic intracellular trafficking.
• Expressed in a variety of tissues including pancreas, heart, leukocyte, spleen, and also in resting and activated peripheral blood T-cells; notably found in breast cancer cells.
• Post-translational modifications include phosphorylation on tyrosine residues and acetylation; alternative splicing and initiation generate multiple isoforms.
• Keywords from UniProt include 3D-structure, phosphoprotein, and reference proteome, underscoring its structural characterization and functional significance.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1-280) of DOK1, which may not detect C-terminally distinct isoforms resulting from alternative initiation; consider validating with isoform-specific panels.
• For Western blotting, the predicted molecular weight is approximately 52 kDa; however, multiple bands may appear due to post-translational modifications and isoform diversity; include appropriate molecular weight markers and positive controls.
• When performing IHC-P or IF/ICC, signal localization to the cytoplasm and perinuclear region is expected; validate staining specificity using relevant knockout or siRNA-treated samples where feasible.
• The antibody is suitable for ELISA; as a polyclonal reagent, it may recognize multiple epitopes, which can enhance sensitivity but may require careful titration to optimize the signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and researchers with reliable access to DOK1-targeting antibodies, recombinant proteins, and ancillary reagents. Our portfolio includes optimized polyclonal and monoclonal antibodies, validated antibody pairs, and bulk reagents to support signal transduction and oncology research. For specialized needs—such as rare target sourcing or custom immunogen design—CamelBio bridges the gap from concept to clinical application.