Anti-DOK1 Monoclonal Antibody for WB, IF/ICC, ELISA - Q99704

Core Product Specifications and Parameters

Biological Background: DOK1 Function and Localization

• DOK1 (docking protein 1), also known as p62(dok) or pp62, is a member of the DOK family of adaptor/scaffold proteins that lack intrinsic enzymatic activity.
• It serves as a docking platform for assembling multimolecular signaling complexes, playing a role in negative regulation of the insulin signaling pathway.
• DOK1 modulates integrin activation by competing with talin for binding to the beta-3 integrin subunit (ITGB3).
• The protein is localized in the cytoplasm, perinuclear region, and nucleus.
• It is expressed in various tissues including pancreas, heart, leukocytes, spleen, and both resting and activated peripheral blood T-cells; also detected in breast cancer.
• Post-translational modifications include phosphorylation and acetylation, with alternative splicing and alternative initiation producing multiple isoforms.
• The protein has a molecular weight of approximately 52 kDa (calculated 52392 Da).

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide from the C-terminal region (amino acids 382-481) of human DOK1, which may confer specificity to the target.
• For Western blot, a band around 52 kDa is expected; validate using appropriate positive and negative controls.
• In immunofluorescence/ICC, anticipate detection in the cytoplasm, perinuclear region, and nucleus, consistent with reported localization.
• When using ELISA, consider the antibody’s performance in sandwich or direct formats and optimize coating and detection conditions.
• Cross-reactivity has been reported with human and mouse; verify reactivity in your experimental system if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supplying high-performance antibodies like this DOK1 rabbit monoclonal antibody for signal transduction research. Beyond this product, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your entire development pipeline from concept to clinic. Whether you need optimized reagents for insulin signaling or integrin biology studies, our one-stop platform streamlines procurement and technical support.