Anti-DOG-1/TMEM16A Rabbit Polyclonal Antibody for WB, ELISA - Q5XXA6

Core Product Specifications and Parameters

Biological Background: DOG-1/TMEM16A Function and Localization

• ANO1 encodes anoctamin-1, a calcium-activated chloride channel (CaCC) that plays central roles in transepithelial anion transport and smooth muscle contraction. Related references: PMID:20056604, PMID:22178883, PMID:22946059
• The channel is indispensable for the pacemaker activity of interstitial cells of Cajal (ICCs) in the gastrointestinal tract, thereby governing gut motility.
• In airway epithelial cells, ANO1 contributes substantially to basal and stimulated chloride conductance and is critical for tracheal cartilage development.
• It facilitates CFTR activation and membrane expression by enhancing endoplasmic reticulum Ca²⁺ store release, underscoring its role in cystic fibrosis-related chloride transport. Related references: PMID:28963502
• ANO1 regulates airway mucus expression induced by interleukins IL3 and IL8 via the asthma-associated protein CLCA1, upregulating MUC5AC; however, conflicting evidence indicates that MUC5AC expression may not require ANO1 in certain contexts. Related references: PMID:33026825, PMID:31732694
• Alternative splicing generates isoforms with distinct calcium sensitivities and chloride permeabilities, including a sweat gland epithelial variant with increased basal Cl⁻ permeability and a hyper-sensitive isoform.
• The protein localizes predominantly to the apical plasma membrane and presynaptic compartments, with broad tissue expression encompassing liver, skeletal muscle, gastrointestinal smooth muscle, kidney collecting ducts, nasal epithelium, and eccrine sweat glands.
• Post-translational modifications, such as glycosylation, disulfide bond formation, and phosphorylation, are critical for anoctamin-1 trafficking and functional regulation.

Experimental Guidance and Technical Tips

• For western blotting, expect a band at approximately 114 kDa under reducing and denaturing conditions; validate using positive control lysates from human or mouse tissues known to express ANO1, such as gastrointestinal smooth muscle or airway epithelium.
• The immunogen (aa 40–210) corresponds to the N-terminal region; detection of certain splice isoforms may vary, so verify isoform-specific recognition if needed.
• When performing ELISA, titrate the antibody to determine optimal working concentrations and confirm specificity against recombinant ANO1 protein or overexpression cell lysates.
• As a polyclonal antibody, lot-to-lot variability is possible; include appropriate controls and consider cross-absorption if non-specific bands are observed.
• Cross-reactivity is verified for human and mouse; for other species, perform sequence alignment of the immunogen region and pilot testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized monoclonal and polyclonal antibodies. For ANO1/DOG-1 studies, we support projects from concept to clinic with bulk ancillary reagents and rare target sourcing. Our integrated supply chain and technical services streamline development for calcium-activated chloride channel research and gastrointestinal stromal tumor diagnostics.