Anti-DOCK7 Polyclonal Antibody for WB, ELISA - Q96N67

Core Product Specifications and Parameters

Biological Background: DOCK7 Function and Localization

• DOCK7 functions as a guanine nucleotide exchange factor (GEF) for Rac1 and Rac3, promoting GDP/GTP exchange; it shows no activity toward CDC42.
• During axon formation, DOCK7 is required for STMN1 phosphorylation at Ser-15, which is critical for neuronal polarization. Related references: PMID:16982419
• As a component of the DISP complex, DOCK7 may regulate septin-actin interactions, thus modulating the actin cytoskeleton. Related references: PMID:29467281
• DOCK7 plays a role in pigmentation, as suggested by similarity to related proteins.
• In cortical development, DOCK7 regulates the proliferation versus differentiation of radial glial cells by negatively regulating TACC3-mediated microtubule growth, controlling interkinetic nuclear migration.
• Subcellularly, DOCK7 localizes to cell projections, particularly axons.
• It is widely expressed across tissues and is subject to multiple post-translational modifications including acetylation, methylation, and phosphorylation.
• DOCK7 is associated with developmental processes, neurogenesis, and epilepsy; alternatively spliced isoforms exist.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 100–200 of human DOCK7 (isoform NP_001354490.1); this region may be conserved across isoforms, potentially allowing recognition of multiple variants.
• For western blotting (WB), the predicted molecular weight of DOCK7 is approximately 243 kDa. Use an appropriate low-percentage SDS-PAGE gel and extended transfer times to ensure efficient detection of such a large protein.
• In ELISA applications, consider using recombinant DOCK7 protein or peptide antigens to validate antibody specificity; direct or indirect ELISA formats can be employed.
• This antibody is documented to cross-react with human, mouse, and rat samples, as indicated by the supplier, but end users should verify reactivity in their specific experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive, one-stop platform for IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For DOCK7-related research in neuronal polarization, cytoskeletal dynamics, and neurodevelopmental disorders, we offer reliable sourcing for anti-DOCK7 polyclonal antibodies like this product, as well as custom development of matched antibody pairs and bulk reagent supply to accelerate your assay development from concept to clinic.