Anti-DOCK3 Rabbit Polyclonal Antibody for WB, ELISA - Q8IZD9

Core Product Specifications and Parameters

Biological Background: DOCK3 Function and Localization

• DOCK3 (Dedicator of Cytokinesis 3), also known as MOCA (Modifier of Cell Adhesion) and PBP (Presenilin-Binding Protein), is a member of the DOCK family of guanine nucleotide exchange factors (GEFs).
• It functions as a potential GEF that activates small GTPases by promoting GDP/GTP exchange, thereby regulating actin cytoskeleton dynamics and cell adhesion.
• DOCK3 interacts with presenilin proteins and stimulates Tau/MAPT phosphorylation, suggesting a role in Alzheimer’s disease pathogenesis.
• Ectopic expression of DOCK3 in nerve cells reduces amyloid-beta secretion and decreases cell-substratum adhesion, linking it to both protein processing and adhesion pathways.
• The protein contains SH3 domains and exhibits SH3-binding motifs, indicating engagement in protein-protein interaction networks.
• Subcellularly, DOCK3 is localized in the cytoplasm, consistent with its role in intracellular signaling.
• In normal brain tissue, DOCK3 is found in the neuropil and occasionally in pyramidal neurons; in Alzheimer’s disease brains, it accumulates in neurofibrillary tangles.
• The DOCK3 gene has been associated with chromosomal rearrangements and disease variants, highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of DOCK3 is approximately 233 kDa; use low-percentage SDS-PAGE gels (e.g., 6%) and extended transfer times for optimal detection.
• The immunogen covers amino acids 1348–1673 of human DOCK3; epitope mapping and validation in relevant cell lines or tissues may be necessary to confirm specificity.
• Given the antibody is raised in rabbit, consider appropriate secondary antibodies and blocking reagents for WB and ELISA.
• In ELISA, the antibody may be used for direct or indirect detection; titrate to determine optimal working concentration in your assay system.
• As cross-reactivity is only reported for human, verify reactivity in other species if needed, particularly in non-human primate models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials. This anti-DOCK3 antibody supports research into Alzheimer’s disease and cytoskeletal dynamics, and through CamelBio’s sourcing services, you can obtain optimized antibody pairs, bulk ancillary reagents, and custom raw materials for your assay development needs.