Anti-DOCK2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q92608

Core Product Specifications and Parameters

Biological Background: DOCK2 Function and Localization

• DOCK2, also known as dedicator of cytokinesis protein 2 or IMD40, is a guanine nucleotide exchange factor (GEF) that catalyzes the release of GDP from monomeric GTPases, facilitating their activation by GTP (PubMed:32651375).
  Related references:
  PMID:32651375

• It specifically activates RAC1 and RAC2 GTPases, but not CDC42, playing a critical role in intracellular signaling (PubMed:21613211, PubMed:32651375).
  Related references:
  PMID:21613211
  PMID:32651375

• DOCK2 is involved in cytoskeletal rearrangements required for lymphocyte migration in response to chemokines, making it essential for immune cell trafficking (PubMed:21613211).
  Related references:
  PMID:21613211

• It may also participate in interleukin-2 (IL2) transcriptional activation via RAC2-mediated pathways (PubMed:21613211).
  Related references:
  PMID:21613211

• The protein localizes to the endomembrane system, cytoplasm, and cytoskeleton, consistent with its role in cell motility and morphology.

• DOCK2 is specifically expressed in hematopoietic cells, with high levels in peripheral blood leukocytes and moderate expression in thymus and spleen; it is expressed at very low levels in the small intestine and colon.

• DOCK2 undergoes post-translational modifications including acetylation and phosphorylation, and contains an SH3 domain, indicating regulatory interactions with other proteins.

• Keywords such as "disease variant" highlight the protein's relevance to immune disorders and potential as a therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1551-1830, corresponding to a C-terminal region of human DOCK2. This region may help generate antibodies recognizing native and denatured protein conformations.
• For Western blot (WB), consider the full-length protein molecular weight of approximately 212 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• Immunofluorescence/immunocytochemistry (IF/ICC) may be used to detect endogenous DOCK2, which can localize to the cytoplasm and cytoskeleton. Include appropriate controls.
• For immunoprecipitation (IP), pre-clearing and using a suitable cross-linker may improve results given the protein's size. Validate the antibody's ability to pull down DOCK2 in your sample system.
• ELISA: consider using a coating buffer and blocking conditions optimized for your antigen. The antibody may be suitable as a detection reagent in sandwich ELISA, but validation in your specific assay is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise covers rare target sourcing and supply for immunology and hematology research, such as DOCK2 and related lymphocyte signaling molecules. From early discovery through commercialization, we offer technical services and consulting to support every stage of your diagnostic development—making CamelBio your reliable partner in bringing novel biomarkers from concept to clinic.