Anti-DOCK1 Rabbit Polyclonal Antibody for WB, ELISA - B2RUU3

Core Product Specifications and Parameters

Biological Background: DOCK1 Function and Localization

• DOCK1 (Aliases: DOCK180, ced5) is a guanine nucleotide exchange factor (GEF) that activates Rac small GTPases, converting them to the GTP-bound active form.
• Contains an SH3 domain and coiled-coil regions, which mediate protein-protein interactions and assembly of signaling complexes.
• Localizes predominantly to the cytoplasm and membrane, facilitating signal transduction at both intracellular and peripheral sites.
• Regulated by phosphorylation, as indicated by its classification as a phosphoprotein.
• The immunogen sequence spans the C-terminal region (amino acids 1766–1865), targeting a conserved domain near the protein’s GEF functional core.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic peptide within the C‑terminal region; verify reactivity in relevant human cell lines or tissues by Western blot at a suggested working dilution optimized empirically.
• For ELISA, a direct or capture format can be explored; pairing with a validated detection antibody may enhance sensitivity.
• As the target is a large membrane/cytoplasmic protein, thorough lysis and adequate blocking conditions are recommended to minimize non‑specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials and technical support. This anti‑DOCK1 antibody complements our portfolio of signal transduction research reagents, and we can support your projects with validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and sourcing of rare‑target biomaterials—helping you move from concept to clinic efficiently.