Anti-DNTTIP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5QJE6

Core Product Specifications and Parameters

Biological Background: DNTTIP2 Function and Localization

• DNTTIP2 (ERBP/TdIF2/LPTS-RP2) is a nuclear protein that regulates transcription by interacting with terminal deoxynucleotidyltransferase (DNTT) and estrogen receptor 1 (ESR1).
  Related references: PMID:12786946, PMID:15047147

• It may function as a chromatin remodeling factor, influencing DNA accessibility and gene expression.
  Related references: PMID:12786946, PMID:15047147

• DNTTIP2 is an integral component of the small subunit (SSU) processome, the earliest precursor of the small ribosomal subunit, where it aids in pre-rRNA maturation.
  Related references: PMID:34516797

• The protein localizes to the nucleus and nucleolus, consistent with its dual roles in transcription regulation and ribosome biogenesis.

• Widely expressed in human tissues, DNTTIP2 shows elevated levels in the testis.

• Post-translational modifications, including phosphorylation and ubiquitin conjugation, are annotated, suggesting regulatory mechanisms.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-300 of human DNTTIP2) covers the N-terminal region; consider its potential to recognize multiple isoforms or cleavage products.
• For Western blot, a band near 84 kDa is expected; validate molecular weight using appropriate positive controls and lysis conditions.
• In immunofluorescence (IF/ICC), nuclear and nucleolar staining should be anticipated; optimize fixation and permeabilization protocols.
• For ELISA applications, the antibody can be utilized with recombinant immunogen as standard; perform cross-validation for your specific assay setup.
• Cross-reactivity with mouse has been demonstrated; verify reactivity in other species by sequence homology alignment before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing solutions, connecting diagnostic manufacturers and research laboratories with high-quality reagents such as this DNTTIP2 antibody. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored rare-target raw-material procurement. Whether your focus is on transcription regulation, ribosome biogenesis, or broader chromatin biology, CamelBio provides the reliable supply chain and technical support to accelerate your project from concept to clinic.