Anti-DNMT3L Polyclonal Antibody for WB, ELISA - Q9UJW3

Core Product Specifications and Parameters

Biological Background: DNMT3L Function and Localization

• DNMT3L is a catalytically inactive DNA methyltransferase-like protein that regulates de novo DNA methylation by interacting with DNMT3A and DNMT3B (PubMed:17687327).
  Related references:
  PMID:17687327
• It recognizes unmethylated histone H3 lysine 4 (H3K4me0) and facilitates DNA methyltransferase activity by accelerating the binding of DNA and S-adenosyl-L-methionine (PubMed:17687327).
  Related references:
  PMID:17687327
• Essential for embryonic stem cell differentiation and germ cell development; required for methylation of imprinted loci and retrotransposon silencing.
• Localizes exclusively to the nucleus, consistent with its role in epigenetic regulation.
• Exhibits tissue-specific expression in testis, ovary, and thymus, with low levels in other tissues.
• Contains a zinc-finger domain and undergoes alternative splicing; identified in proteomics studies as a nuclear protein.
• Functions bimodally: promotes DNA methylation at housekeeping genes but inhibits methylation at bivalent gene promoters by interacting with the PRC2/EED-EZH2 complex.

Experimental Guidance and Technical Tips

• For western blotting, consider using nuclear extracts from cells known to express DNMT3L (e.g., testis or embryonic stem cells). The antibody detects a band at approximately 44kDa; validate molecular weight against appropriate markers.
• As a polyclonal antibody, some non-specific bands may appear; titrate dilution and include proper controls.
• In ELISA, the immunogen corresponds to a large portion of the protein, enabling sensitive detection of recombinant or native DNMT3L. Optimize coating and detection conditions.
• Confirm reactivity in human and mouse samples, but verify performance in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, technical services, and consulting. For DNMT3L-related epigenetic research or assay development, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare target raw materials. Trust CamelBio as your reliable partner from concept to clinic.