Anti-DNMT3B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBC3

Core Product Specifications and Parameters

Biological Background: DNMT3B Function and Localization

• Encodes DNA (cytosine-5)-methyltransferase 3B (DNMT3B), also known as DNA methyltransferase HsaIIIB (M.HsaIIIB), with multiple isoforms; isoforms 4 and 5 lack conserved methyltransferase motifs and are likely non-functional.
• Essential for genome-wide de novo DNA methylation during development, establishing methylation patterns coordinated with histone methylation (By similarity).
• Preferentially methylates nucleosomal DNA within the nucleosome core region and may function as a transcriptional co-repressor by associating with CBX4 or ZHX1 independently of its catalytic activity (By similarity).
• Required for DUX4 silencing in somatic cells, highlighting its role in gene repression (PubMed:27153398). Related references: PMID:27153398
• Subcellularly localized to the nucleus, consistent with its function in chromatin modification.
• Ubiquitously expressed with highest levels in fetal liver, heart, kidney, and placenta; specific isoforms show tissue-restricted patterns (e.g., isoform 5 is testis-specific).
• Post-translationally modified by phosphorylation, citrullination, and ubiquitination; contains zinc-finger domains and is a S-adenosyl-L-methionine-dependent methyltransferase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 345-475 of human DNMT3B, a region within the catalytic methyltransferase domain; this suggests the antibody may recognize both full-length and truncated isoforms that retain this region.
• For Western blot (WB), the predicted molecular weight of the canonical isoform is 96 kDa, but other isoforms and post-translational modifications may produce additional bands; consider using nuclear extracts from human, mouse, or rat tissues/cell lines.
• For immunofluorescence (IF/ICC), nuclear staining is expected; validate with appropriate nuclear counterstains and controls in relevant cell systems.
• For ELISA, perform standard titration and specificity verification using the recombinant immunogen or full-length protein as positive control.
• Due to the polyclonal nature, batch-to-batch consistency should be verified with appropriate positive and negative controls in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one-stop IVD raw material partner, CamelBio helps diagnostic developers and researchers accelerate their projects from concept to clinic. For epigenetics and DNA methylation research, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, ancillary reagents, and specialized sourcing for rare targets. We are dedicated to streamlining your supply chain with reliable, high-quality materials and technical support.