Anti-DNMT3B Polyclonal Antibody for WB, IHC-P, ELISA - Q9UBC3

Core Product Specifications and Parameters

Biological Background: DNMT3B Function and Localization

• DNMT3B (DNA (cytosine-5)-methyltransferase 3B) is a key enzyme required for genome-wide de novo DNA methylation, essential for establishing methylation patterns during early development.
• It preferentially methylates nucleosomal DNA within the core region and can function as a transcriptional co-repressor by interacting with CBX4 and ZHX1, independently of its methyltransferase activity.
• In a complex with DNMT1 and the transcription factor CTCFL/BORIS, DNMT3B activates BAG1 gene expression through modulation of histone H3 dimethylation at K4 and K9.
• Required for DUX4 silencing in somatic cells (PubMed:27153398). Related references: PMID:27153398
• Alternative splicing generates several isoforms; isoforms 4 and 5 lack critical methyltransferase motifs and are likely non-functional.
• DNMT3B is exclusively nuclear and is ubiquitously expressed, with highest levels in fetal liver, heart, kidney, and placenta. Isoform 1 is absent in brain, skeletal muscle, and PBMC, while isoform 5 is testis-specific.
• The protein contains zinc-finger domains, undergoes phosphorylation and citrullination, and plays roles in gene silencing and disease (e.g., ICF syndrome).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein corresponding to the N-terminal region (aa 1-100) of human DNMT3B. This region is present in most isoforms; however, confirm the detection of all expected variants in your sample.
• For Western blotting, the predicted molecular weight is ~96 kDa. Use nuclear extracts or whole-cell lysates from tissues with high DNMT3B expression (e.g., fetal liver or placenta) for optimal detection.
• This antibody is reported to cross-react with mouse and rat; validate performance in the relevant species and application.
• For immunohistochemistry (IHC-P), consider testing on fetal tissue sections where DNMT3B is abundant. Antigen retrieval conditions may require optimization.
• ELISA applications should include proper negative and positive controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for epigenetic and DNA methylation research. For rare or custom targets like DNMT3B, we offer specialized sourcing and supply chain solutions to meet your development needs.