Anti-DNMT3A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6K1

Core Product Specifications and Parameters

Biological Background: DNMT3A Function and Localization

• DNMT3A is a DNA (cytosine-5)-methyltransferase essential for de novo DNA methylation, establishing methylation patterns during development and coordinating with histone modifications (PubMed:12138111, PubMed:16357870, PubMed:30478443).
  Related references: PMID:12138111, PMID:16357870, PMID:30478443
• It modifies DNA in a non-processive manner and methylates non-CpG sites, with a preference for DNA linker regions between nucleosomes, and its activity is inhibited by histone H1 (By similarity).
• DNMT3A plays a critical role in paternal and maternal imprinting and is required for methylation of most imprinted loci in germ cells (By similarity).
• It acts as a transcriptional corepressor for ZBTB18, is recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3), and can repress transcription through recruitment of HDAC activity (By similarity).
• The protein localizes mainly to the nucleus and chromosomes, but is also detectable in the cytoplasm (UniProt Q9Y6K1).
• Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, and kidney, with lower levels in placenta, brain, liver, colon, spleen, small intestine, and lung (UniProt Q9Y6K1).
• Alternative promoter usage and splicing produce isoforms such as DNMT3A2 (TBRS, HESJAS), adding functional diversity (UniProt Q9Y6K1).

Experimental Guidance and Technical Tips

• For Western blot, consider using a nuclear extraction protocol to enrich DNMT3A signal, expecting a band around 102kDa. Validate antibody specificity with DNMT3A knockout or siRNA-treated samples.
• For immunofluorescence/immunocytochemistry (IF/ICC), anticipate predominantly nuclear staining, with possible cytoplasmic signal in some cell types; use appropriate fixation and permeabilization methods.
• In ELISA applications, titrate the antibody against recombinant DNMT3A protein or peptide antigen to optimize working concentrations.
• When assessing cross-reactivity across human, mouse, and rat, confirm sequence homology in the immunogen region to ensure accurate detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers with validated antibody pairs, optimized polyclonal antibodies like this anti-DNMT3A, and bulk ancillary reagents for epigenetic research. Our integrated supply chain capabilities also extend to rare target raw-material procurement, helping to streamline your development from concept to clinic.