Anti-DNMT3A Rabbit Monoclonal Antibody for WB, ELISA - Q9Y6K1

Core Product Specifications and Parameters

Biological Background: DNMT3A Function and Localization

• DNMT3A is a de novo DNA methyltransferase required for genome-wide methylation patterning during development. It coordinates DNA and histone methylation, and modifies non-CpG sites in a non-processive manner.
  Related references: PMID:12138111, PMID:16357870, PMID:30478443
• May preferentially methylate DNA linker between two nucleosomal cores; inhibited by histone H1 (By similarity).
• Plays a role in paternal and maternal imprinting and is required for methylation of most imprinted loci in germ cells (By similarity).
• Acts as a transcriptional corepressor for ZBTB18, recruited to trimethylated H3K36 (H3K36me3), and can repress transcription through HDAC recruitment (By similarity).
• Exhibits weak auto-methylation activity on Cys-710 in the absence of DNA (By similarity).
• Subcellular localization is predominantly nuclear, with additional presence in chromosome and cytoplasm.
• Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, and kidney; lower levels in placenta, brain, liver, colon, spleen, small intestine, and lung.
• Disease-associated variants link DNMT3A to Tatton-Brown-Rahman syndrome (dwarfism and intellectual disability) and hematological malignancies such as acute myeloid leukemia. Alternative splicing produces isoforms like DNMT3A2.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 550–650 of human DNMT3A; consider epitope accessibility when planning immunoassays.
• This antibody is suitable for Western blot (WB) and ELISA. For WB, the expected molecular weight of DNMT3A is approximately 102 kDa.
• Reported as KO validated; verify specificity using appropriate knockout or knockdown controls in your target system.
• Currently validated for human reactivity; cross-reactivity with other species has not been assessed.
• Optimal working concentrations for WB and ELISA should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing antibodies, antigens, and ancillary reagents tailored to diagnostic development and research. The DNMT3A rabbit monoclonal antibody supports epigenetic research, particularly DNA methylation studies, with applications in WB and ELISA. With capabilities in validated antibody pairs, custom monoclonal/polyclonal development, and rare-target sourcing, CamelBio helps streamline your assay pipeline from concept to clinic.