Anti-DNMT1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P26358

Core Product Specifications and Parameters

Biological Background: DNMT1 Function and Localization

• DNMT1, also known as DNA methyltransferase HsaI, MCMT, or CXXC9, is a ~183 kDa nuclear protein that serves as the primary maintenance DNA methyltransferase in humans.
• It preferentially methylates hemimethylated CpG dinucleotides, and associates with DNA replication foci during S phase to faithfully copy methylation patterns onto the newly synthesized strand, ensuring epigenetic inheritance. Related references: PMID:17200670, PMID:21745816, PMID:26070743
• During G2 and M phases, DNMT1 remains chromatin-associated to sustain methylation independently of replication, thereby preserving genomic methylation marks across the cell cycle. Related references: PMID:21745816
• DNMT1 also mediates transcriptional repression by directly binding HDAC2 and can coordinate DNA methylation with histone modifications, such as H3K9 dimethylation, in concert with DNMT3B and CTCFL. Related references: PMID:10888872, PMID:18413740
• It forms a corepressor complex that facilitates KRAS-driven hypermethylation and silencing of tumor suppressor genes in colorectal cancer, and similarly maintains repression of developmental genes in embryonic stem cells. Related references: PMID:24623306
• Ubiquitously expressed with highest levels in fetal tissues, heart, kidney, and placenta; isoform 2 is less abundant. Localizes to the nucleus and chromosomes, and contains characteristic CXXC zinc-finger domains (Keywords: alternative splicing, zinc-finger).

Experimental Guidance and Technical Tips

• The antibody was raised against an N-terminal fragment (aa 1-124) of DNMT1; therefore, it is expected to recognize full-length DNMT1 and possibly N-terminal truncation products.
• When performing western blot, use a 6-8% SDS-PAGE gel and ensure efficient transfer due to the large size of DNMT1 (~183 kDa).
• For immunohistochemistry and immunofluorescence, nuclear permeabilization may be required; consider validating staining specificity with DNMT1 knockdown or knockout controls.
• The antibody cross-reacts with mouse and rat DNMT1, but users should verify performance in each species and tissue type given the ubiquitous but variable expression.
• As a polyclonal antibody, it may exhibit lot-to-lot variability; include a loading control and internal reference standards for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated primary antibodies, antibody pairs, and ancillary reagents. This anti-DNMT1 polyclonal antibody supports epigenetic research and oncology biomarker studies, and CamelBio can further assist with sourcing rare-target reagents, custom immunological development, and bulk supply needs. Reach out to our team for tailored technical consultation and procurement solutions.