Anti-DNMT1 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P26358

Core Product Specifications and Parameters

Biological Background: DNMT1 Function and Localization

• Protein Identity and Isoforms: DNMT1 (DNA (cytosine-5)-methyltransferase 1) is a CXXC-type zinc finger protein with aliases MCMT, CXXC9, and DNA methyltransferase HsaI. Multiple isoforms exist; isoform 2 is less expressed than the canonical isoform 1.
• Maintenance DNA Methylation: Functions as the primary maintenance DNA methyltransferase, preferentially methylating hemimethylated CpG sites to preserve methylation patterns during DNA replication. Associates with replication foci in S phase. Related references: PMID:17200670, PMID:21745816, PMID:26070743
• Chromatin Association during Cell Cycle: In addition to S-phase association, DNMT1 binds chromatin during G2 and M phases, contributing to replication-independent maintenance of DNA methylation. Related references: PMID:21745816
• Transcriptional Repression: Mediates transcriptional repression through direct interaction with HDAC2, and coordinates DNA methylation with histone modifications to regulate gene expression. Related references: PMID:10888872, PMID:16357870
• Dual Role in Gene Silencing and Activation: Forms corepressor complexes that promote KRAS-mediated hypermethylation and silencing of tumor suppressor genes in colorectal cancer cells; also maintains repressive chromatin states in embryonic stem cells. Conversely, it can participate in gene activation (e.g., BAG1) through modulation of histone H3K4 and H3K9 dimethylation in complex with DNMT3B and CTCFL/BORIS. Related references: PMID:24623306, PMID:18413740
• Subcellular Localization and Tissue Expression: Predominantly nuclear, associated with chromosomes. Ubiquitously expressed; highly abundant in fetal tissues, heart, kidney, placenta, and peripheral blood mononuclear cells, with lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle.
• Post-translational Modifications and Disease Associations: Subject to acetylation, phosphorylation, and ubiquitination. Mutations in DNMT1 are linked to hereditary sensory neuropathy and autosomal dominant cerebellar ataxia, deafness, and narcolepsy (ADCADN).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–100 at the N-terminus of human DNMT1; consider using a peptide blocking assay to confirm specificity if needed.
• For Western blot, the predicted molecular weight is ~183 kDa. Use a low-percentage acrylamide gel (e.g., 6–8%) and optimize transfer conditions for high-molecular-weight proteins.
• In IHC-P, perform appropriate antigen retrieval and validate staining using positive and negative tissue controls (e.g., heart, placenta, or colon).
• The antibody is reported to cross-react with human, mouse, and rat samples. When extending to other species, verify reactivity experimentally.
• For immunoprecipitation and ELISA, follow standard protocols and include proper controls to account for potential non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw material solutions, connecting diagnostic manufacturers and research laboratories with validated antibodies like this DNMT1 monoclonal antibody for DNA methylation and epigenetic studies. Beyond single antibodies, we offer antibody pairs, bulk ancillary reagents, and custom sourcing for rare or difficult-to-obtain targets. Let CamelBio be your reliable partner from concept to clinic, streamlining your procurement and accelerating your assay development.