Anti-DNM2 Polyclonal Antibody for IF/ICC, ELISA - P50570

Core Product Specifications and Parameters

Biological Background: DNM2 Function and Localization

• Encodes dynamin-2, a large GTPase that catalyzes GTP hydrolysis to power membrane scission during endocytosis and actin filament remodeling. Related references: PMID:15731758, PMID:19605363, PMID:19623537

• Self-assembles into helical ring‑like structures around vesicle necks; GTP hydrolysis then drives rapid disassembly, enabling vesicle release. Related references: PMID:17636067, PMID:34744632, PMID:36445308

• Participates in multiple trafficking routes: clathrin‑mediated endocytosis, exocytosis from the trans‑Golgi network, and PDGF‑stimulated macropinocytosis. Related references: PMID:33713620

• Involved in autophagy by forming ATG9A vesicles and mediating recycling endosome scission for autophagosome release. Related references: PMID:29437695, PMID:32315611

• Localizes to cytoplasm, cytoskeleton, clathrin‑coated vesicles, recycling endosomes, phagosomes, podosomes, centrosomes, midbody, postsynaptic density, and synaptosomes.

• Widely expressed, with notable presence in skeletal muscle and peripheral nerve. Related references: PMID:7590285, PMID:19623537

• Acts as a motor protein linking membranes to the actin cytoskeleton; mutations are associated with Charcot‑Marie‑Tooth disease (CMT) subtypes, including CMT2M and CMTDIB.

• Subject to post‑translational modifications such as acetylation and phosphorylation, which may regulate its oligomerization, localization, and activity.

Experimental Guidance and Technical Tips

• The immunogen (aa 607‑866) spans the GTPase effector domain (GED) and a portion of the proline‑rich domain; this design may provide specificity for DNM2 by targeting regions involved in self‑assembly and protein interactions.

• For IF/ICC detection, permeabilization is essential to access cytoplasmic, vesicular, and cytoskeletal DNM2 pools. Include isotype or blocking‑peptide controls to confirm signal specificity.

• For ELISA, consider using recombinant full‑length DNM2 or lysates from relevant tissues (e.g., muscle, nerve) as coating antigen, and titrate the antibody to optimize signal‑to‑noise ratio.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supplies a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. For targets like DNM2—critical in endocytosis, actin dynamics, and CMT‑linked neuropathies—we can source rare raw materials to support your research and assay pipelines. Partner with us to streamline your transition from concept to clinic with reliable, one‑stop access to the tools you need.