Anti-DNM1 Rabbit Polyclonal Antibody for WB and ELISA - Q05193

Core Product Specifications and Parameters

Biological Background: DNM1 Function and Localization

• Dynamin-1 (DNM1) is a large GTPase with a molecular weight of ~97 kDa (UniProt: 97,408 Da) that plays a central role in membrane fission during endocytosis.
• Catalyzes the hydrolysis of GTP to mediate vesicle scission and participates in multiple endocytic pathways, including clathrin-mediated endocytosis and synaptic vesicle endocytosis (PubMed:15703209, PubMed:20428113, PubMed:29668686). Related references: PMID:15703209 PMID:20428113 PMID:29668686
• Self-assembles into rings and helical polymers around vesicle necks, constricting the membrane; GTP hydrolysis then triggers conformational changes that promote membrane fission (PubMed:30069048, PubMed:7877694, PubMed:9922133, PubMed:19084269). Related references: PMID:30069048 PMID:7877694 PMID:9922133 PMID:19084269
• Regulates early stages of clathrin-mediated endocytosis in non-neuronal cells via dephosphorylation downstream of EGFR signaling, and interacts with DNAJC6 during clathrin-coated vesicle (CCV) formation (PubMed:29668686, PubMed:12791276). Related references: PMID:29668686 PMID:12791276
• Mediates rapid endocytosis (RE), a Ca²⁺-dependent, clathrin- and K⁺-independent process in chromaffin cells; also associates with microtubules and produces force (By similarity).
• Subcellularly localizes to the cell membrane, clathrin-coated pits, cytoplasmic vesicles, presynapse, and chromaffin granules.
• Subject to multiple post-translational modifications, including phosphorylation, methylation, and nitration; alternative splicing generates multiple isoforms. Keywords also highlight involvement in disease variants and epilepsy.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 400–490 of human DNM1, a central region. Verify sequence homology if the antibody is intended for use in other species.
• For Western blotting, DNM1 migrates at approximately 97 kDa under denaturing conditions; include appropriate molecular weight markers and validate with positive control lysates from human, mouse, or rat brain or neuronal cell lines.
• For ELISA, consider using the recombinant immunogen or full-length DNM1 as coating antigen; perform checkerboard titration to optimize working concentrations of capture and detection reagents.
• Cross-reactivity is reported for human, mouse, and rat; however, individual experimental systems may require optimization of blocking and antibody dilution conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and clinical institutions with a streamlined supply chain for IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our DNM1 antibody supports neuroscience and cell biology research focused on endocytosis, synaptic transmission, and neurological disorders. Contact us for technical consultation, customized sourcing, and all ancillary reagent needs to accelerate your assay development from concept to clinic.